海恩酮 - CAS:3105-97-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	海恩酮
英文名称:	Hycanthone
别名:	羟甲硫蒽酮；羟胺硫蒽酮 1-((2-(Diethylamino)ethyl)amino)-4-(hydroxymethyl)-9H-thioxanthen-9-one
CAS No:	3105-97-3	分子式:	C₂₀H₂₄N₂O₂S	分子量:	356.48
CAS No:	3105-97-3
分子式:	C₂₀H₂₄N₂O₂S
分子量:	356.48
EINEC:	221-463-7
EINEC:	221-463-7

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

H10302 海恩酮 ≥98% (psaitong)

相关产品

包装规格：

10mg in glass bottle

溶解性：

溶于DMSO（12.5 mg/mL 超声)

产品描述：

基本信息

产品编号：H10302
产品名称：Hycanthone
CAS:	3105-97-3	储存条件	粉末	-20℃	四年

分子式：	C20H24N2O2S		溶于液体	-80℃	六个月
分子量	356.48			-20℃	一个月
化学名：	1-((2-(Diethylamino)ethyl)amino)-4-(hydroxymethyl)-9H-thioxanthen-9-one
Solubility (25°C)	体外	DMSO	10mg/mL (28.05mM; Need ultrasonic)
		Ethanol
		Water
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.8052mL	14.0260mL	28.0521mL
5mM	0.5610mL	2.8052mL	5.6104mL
10mM	0.2805mL	1.4026mL	2.8052mL

生物活性

产品描述	一种噻吨酮类的 DNA 嵌入剂 (DNA intercalator)，可抑制 RNA 合成以及 DNA 拓扑异构酶 I 和 II。
靶点/IC₅₀	Topoisomerase I	Topoisomerase II
体外研究	Hycanthone has an IC50 of 80nM for inhibition of APE1 incision of depurinated plasmid DNA. Hycanthone (0.05-100 µM; for 2 h) promotes APE1 cleavage in presence of Cycloheximide (CHX) and this cleavage is inhibited by 1% DMSO. Hycanthone at 20mg/mL or more is progressively more detrimental to cell viability. Results reveal that increased concentrations of Hycanthone, ranging from 0.1 to 10μg/mL, progressively reduces viral interferon yields as much as 73% compare to that of controls.
体内研究	Results show that the incorporation of tritiated thymidine into TCA-precipitable material of adult sensitive worms undergo a progressive decrease after treatment with Hycanthone. Immature worms are totally unaffected by Hycanthone at all times tested. Male worms treated with Hycanthone show signs of a possible partial recovery from the initial low levels of incorporation. The incorporation of tritiated leucine by drug-sensitive worms treated with Hycanthone is inhibited by 40 to 50% in the first four days after treatment. Results show that, 7 days after Hycanthone treatment, both ribosomal RNA species are reduced by at least 80% with respect to untreated worms, with some indication of a possible accumulation of heavier precursor molecules.

推荐实验方法(仅供参考)

细胞实验：

Cell Assay

Appropriate quantities of Hycanthone (1 to 100μg) in 10mL maintenance medium are added to plastic flasks (75cm2) containing approximately 3×10⁷LLC-MK2 cells in monolayer, which are then incubated at 35°C for 24h. Maintenance medium is decanted, and 2mL influenza virus is added onto cell monolayers and incubated at 35°C for 2h. The multiplicity of infection is approximately 1.0. Inoculum is removed and 10mL maintenance medium is added to each flask, which is then incubated at 35°C for 24h. Supernatant fluid is decanted, centrifuged at 100,000 g for 1 h, dialyzed against HCI-KCI buffer (pH 2.0) at 4°C for 24 h, and then dialyzed against two changes of phosphate-buffered saline (pH 7.1) at 4°C for 24h. Fluids are passed through filters to obtain sterile preparations. Samples are stored at -80°C until assayed for interferon activity.

动物实验:
Animal Administration	Female outbred Swiss albino mice used as definitive hosts weigh 18 to 20 g at the time of infection. Hycanthone is administered at 0.01mL/g body weight intramuscularly by splitting the dose into the two hind legs, so that each mouse receives 80mg/kg body weight of the free base. Treatments are usually performed during the 8th week after infection.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果