中文名称: | Flagelin 22 TFA | ||||
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英文名称: | Flagelin 22 TFA | ||||
别名: | FLG22 Flagellin 22 TFA | ||||
CAS No: | 分子式: | C95H163F3N32O36 | 分子量: | 2386.5 | |
CAS No: | |||||
分子式: | C95H163F3N32O36 | ||||
分子量: | 2386.5 |
基本信息
产品编号:F10194 |
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产品名称:Flagelin 22 TFA |
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CAS: |
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储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
2386.50 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
200mg/mL (83.80mM Need ultrasonic) |
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Ethanol |
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Water |
≥50mg/mL(20.95mM) |
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体内 |
现配现用 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
0.4190mL |
2.0951mL |
4.1902mL |
5mM |
0.0838mL |
0.4190mL |
0.8380mL |
10mM |
0.0419mL |
0.2095mL |
0.4190mL |
生物活性
产品描述 |
细菌鞭毛素的一个片段,在植物和藻类中都是有效的诱导剂。 |
靶点/IC50 |
Bacterial |
体外研究 |
Flagelin 22 (flg22) is a 22-amino-acid peptide, which corresponds to the highly conserved N-terminal region of flagellin, can induce immunity reaction in various plants such as tomato (Solanum lycopersicum), potato (Solanum tuberosum), tobacco (Nicotiana tabacum), and Arabidopsis thaliana. Flagelin 22 can induce oxidative bursts and hypersensitive responses (HR) in both female gametophytes and sporophytes of Saccharina japonica, indicating that algae and plants may share similar mechanisms for recognizing pathogens. After culturing the female gametophytes of S. japonica in the presence of Flagelin 22, flg15, flg14, and flg22D43A for 40 days, both Flagelin 22 and flg15 significantly induce growth inhibition of the algae at a concentration of 1μM. The fresh weights of Flagelin 22- and flg15-challenged female gametophytes are less than one half of the control. |
推荐实验方法(仅供参考)
细胞实验: |
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Cell Assay |
One milliliter of the female gametophytes is added to 100mL sterilized seawater containing 1μM of Flagelin 22, flg15, flg14,and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50μmol photons m-2 s-1. Sterilized seawater medium is provided with 0.2mM KNO3,0.02mM KH2PO4, and 1μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )