| 中文名称: | 法罗培南钠 | ||||
|---|---|---|---|---|---|
| 英文名称: | faropenem sodium | ||||
| 别名: | 法罗培南钠 (5R,6S)-6-(1(R)-Hydroxyethyl)-2-(2(R)-tetrahydrofuryl)penem-3-carboxylic acid monosodium salt | ||||
| CAS No: | 122547-49-3 | 分子式: | C12H14NNaO5S | 分子量: | 307.3 |
| CAS No: | 122547-49-3 | ||||
| 分子式: | C12H14NNaO5S | ||||
| 分子量: | 307.3 | ||||
包装规格：
5g 25g 100g in glass bottle
产品简介：
Faropenem sodium 是一种可有效杀死结核分枝杆菌的口服可吸收的培南抗生素。
溶解性：
溶于DMSO（25 mg/mL 超声)
储备液保存：
-80°C, 6 months; -20°C, 1 month
体内实验：
1、请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (325.41 mM); Clear solution; Need ultrasonic
2、请依序添加每种溶剂： 10% DMSO→40% PEG300→5% Tween-80 →45% saline
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
3、请依序添加每种溶剂： 10% DMSO→90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
4、请依序添加每种溶剂： 10% DMSO→90% corn oil
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
Solubility: 100 mg/mL (325.41 mM); Clear solution; Need ultrasonic
2、请依序添加每种溶剂： 10% DMSO→40% PEG300→5% Tween-80 →45% saline
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
3、请依序添加每种溶剂： 10% DMSO→90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
4、请依序添加每种溶剂： 10% DMSO→90% corn oil
Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.14 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
体外研究：
Faropenem is an orally bioavailable (72 to 84% bioavailability) penem antibiotic that is more resistant to hydrolysis by β-lactamases than cephalosporins and carbapenems are. It efficiently inactivates M. tuberculosis l,d-transpeptidases and exhibits antimycobacterial activity in vitro and in macrophages.
细胞实验：
细胞系：RAW macrophages
浓度：7, 28, or 56 μg/ml
处理时间：--
方法：RAW macrophages were grown in Dulbecco modified Eagle medium containing l-glutamine and sodium pyruvate (PAA Laboratories) supplemented with 10% fetal bovine serum. About 2.5 × 104 cells were seeded into each well of a 48-well plate and were infected with green fluorescent protein (GFP)-expressing M. tuberculosis (MTB_NDT1) at a multiplicity of infection (MOI) of 10:1 for 4 h. After infection, the monolayers were washed three times with fresh medium to remove nonadherent bacteria and then exposed to fresh medium with or without isoniazid (0.75 μg/ml), faropenem (7, 28, or 56 μg/ml), or meropenem (5, 25, or 50 μg/ml) plus clavulanate (2.5 μg/ml). Medium (containing antibiotics) was changed every 24 h. At various time points, macrophages were washed once with 1× PBS and then lysed using 0.5% Triton X-100. The macrophage lysates were diluted in PBS containing 0.05% Tween 80, serial dilutions were plated on 7H10 agar, and bacterial CFU were enumerated after incubation of the plates at 37°C for 3 to 4 weeks. For analysis of the infected macrophages by flow cytometry, the cells were washed once with 1× PBS before being resuspended in PBS. Cells were analyzed by flow cytometry using a BD Accuri C6 flow cytometer. The threshold was set at 80,000, gates were drawn to exclude clumps and debris, and 50,000 gated events were acquired for each sample. RAW macrophages infected with nonfluorescent M. tuberculosis were used to determine the background fluorescence gates. The fraction of infected cells was calculated from the number of events exhibiting fluorescence above this background level.
浓度：7, 28, or 56 μg/ml
处理时间：--
方法：RAW macrophages were grown in Dulbecco modified Eagle medium containing l-glutamine and sodium pyruvate (PAA Laboratories) supplemented with 10% fetal bovine serum. About 2.5 × 104 cells were seeded into each well of a 48-well plate and were infected with green fluorescent protein (GFP)-expressing M. tuberculosis (MTB_NDT1) at a multiplicity of infection (MOI) of 10:1 for 4 h. After infection, the monolayers were washed three times with fresh medium to remove nonadherent bacteria and then exposed to fresh medium with or without isoniazid (0.75 μg/ml), faropenem (7, 28, or 56 μg/ml), or meropenem (5, 25, or 50 μg/ml) plus clavulanate (2.5 μg/ml). Medium (containing antibiotics) was changed every 24 h. At various time points, macrophages were washed once with 1× PBS and then lysed using 0.5% Triton X-100. The macrophage lysates were diluted in PBS containing 0.05% Tween 80, serial dilutions were plated on 7H10 agar, and bacterial CFU were enumerated after incubation of the plates at 37°C for 3 to 4 weeks. For analysis of the infected macrophages by flow cytometry, the cells were washed once with 1× PBS before being resuspended in PBS. Cells were analyzed by flow cytometry using a BD Accuri C6 flow cytometer. The threshold was set at 80,000, gates were drawn to exclude clumps and debris, and 50,000 gated events were acquired for each sample. RAW macrophages infected with nonfluorescent M. tuberculosis were used to determine the background fluorescence gates. The fraction of infected cells was calculated from the number of events exhibiting fluorescence above this background level.
动物实验：
动物模型：Adult female C57BL/6J mice
剂量：500 mg/kg
给药处理：oral
剂量：500 mg/kg
给药处理：oral
注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。
2、以上信息仅做参考交流之用。
2、以上信息仅做参考交流之用。
保存条件：
-20℃
UN码：
HazardClass：
危害声明：
安全说明：
搜索质检报告(COA)
参考文献 & 客户发表文献
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
