| 中文名称: | EIPA 促销 热销 | ||||
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| 英文名称: | EIPA | ||||
| 别名: | EIPA L593754;MH12-43 | ||||
| CAS No: | 1154-25-2 | 分子式: | C11H18ClN7O | 分子量: | 299.76 |
| CAS No: | 1154-25-2 | ||||
| 分子式: | C11H18ClN7O | ||||
| 分子量: | 299.76 | ||||
基本信息
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产品编号： |
E10484 |
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产品名称： |
EIPA |
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CAS: |
1154-25-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
299.76 |
-20℃ |
1个月 |
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化学名： |
3-amino-6-chloro-N-(diaminomethylidene)-5-[ethyl(propan-2-yl)amino]pyrazine-2-carboxamide |
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Solubility (25°C)： |
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体外:
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DMSO |
60mg/mL |
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Ethanol |
30mg/mL |
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Water |
2mg/mL |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.3360mL |
16.6800mL |
33.3600mL |
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5mM |
0.6672mL |
3.3360mL |
6.6720mL |
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10mM |
0.3336mL |
1.6680mL |
3.3360mL |
生物活性
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产品描述 |
一种TRPP3channel抑制剂，其IC50值为10.5μM。EIPA还抑制Na+/H+交换(NHE)和巨噬细胞。 |
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靶点 |
IC50:10.5μM (TRPP3 channel) NHE Macropinocytosis |
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体外研究 |
In the presence of 100μM EIPA,10μM benzamil,and 10μM phenamil,45Ca2+uptake decreases from 79±9 to 46±4 (58% remaining),27±4 (34%),29±5 (37%),and 38±4 (48%) pmol/oocyte/30 min (n=6,P=0.008),respectively.It is found that EIPA,benzamil,and phenamil rapidly and reversibly block Ca2+-activated TRPP3 channel activation at -50mV,with IC50s of 143±8 (n=36),10.5±2.2 (n=28),1.1±0.3 (n=30),and 0.14±0.04μM (n=25),respectively.The number of autophagic vacuoles increases dramatically in the HAE and HPE groups after EIPA treatment compare with the HAN and HPN groups.EIPA regulates the initiation and maturation of the autophagy associated with amino acids in IEC-18 cells.In addition,the uptake of cinnamoylphenazine (CA-PZ) and neutral red (NR) is inhibited by EIPA. |
推荐实验方法(仅供参考)
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细胞实验: |
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The effect of EIPA alone (without alanine or proline) is also examined in both control (DMEM cultured cells) and amino acidstarved cells.The cells are incubated for 6h in DMEM containing 5% FBS and either 0 or 0.3mM EIPA (labelled QNN and QNE,respectively),HBSS containing either 0 or 0.3mM EIPA (labelled HNN and HNE,respectively),HBSS with 1.0mM alanine (labelled HAN) or 0.5mM proline (labelled HPN),HBSS with 1.0mM alanine and 0.3mM EIPA (labelled HAE),and HBSS with 0.5mM proline and 0.3mM EIPA (labelled HPE). |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
