| 中文名称: | Episilvestrol | ||||
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| 英文名称: | Episilvestrol | ||||
| 别名: | Episilvestrol 国华试剂 methyl (1R,2R,3S,3aR,8bS)-6-[[(2S,3R,6R)-6-[(1S)-1,2-dihydroxyethyl]-3-methoxy-1,4-dioxan-2-yl]oxy]-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate | ||||
| CAS No: | 697235-39-5 | 分子式: | C34H38O13 | 分子量: | 654.66 |
| CAS No: | 697235-39-5 | ||||
| 分子式: | C34H38O13 | ||||
| 分子量: | 654.66 | ||||
基本信息
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产品编号： |
E10375 |
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产品名称： |
Episilvestrol |
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CAS: |
697235-39-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
654.66 |
-20℃ |
一个月 |
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化学名： |
(1S,2R,3R,3aS,8aR)-6-{(2S,3R,6R)-6-[(S)-1,2-dihydroxy-ethyl]-3-methoxy-[1,4]dioxan-2-yloxy}-3,3a-dihydroxy-4-methoxy-8a-(4-methoxy-phenyl)-1-phenyl-2,3,3a,8a-tetrahydro-1H-8-oxa-cyclopenta[a]indene-2-carboxylic acid methyl ester |
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生物活性
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产品描述 |
silvestrol 的衍生物，从 Aglaia silvestris 中分到，能够抑制 eIF4A 靶作用的转录过程。 |
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靶点 |
eIF4A |
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体外研究 |
Episilvestrol is a specific eIF4A-targeting translation inhibitor, with antitumor activity. Episilvestrol is cytotoxic activity against several human cancer cell lines, such as Lu1, LNCaP, MCF-7 and HUVEC cells, with ED50s of 3.8, 3.8, 5.5 and 15.3nM, respectively. The GI50s of Episilvestrol against the cell proliferation of NCI-H460 and MCF-7 cels are 17.96nM and 17.96nM after first test and 15.6nM and 18.7nM after 2 months via SRB assay. Episilvestrol also suppresses HK1 cells and EBV-positive C666.1 NPC cells |
推荐实验方法(仅供参考)
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Cell Assay |
A total of 1×104 HK1 cells/well or 3×104 C666.1 cells/well are seeded into 96-multiwell microtiter plates. At 24h following seeding, the medium is aspirated and replaced with fresh medium containing various concentrations of silvestrol or Episilvestrol. Vehicle control cultures receive DMSO alone. The cells are then incubated for 24h at 37°C in an atmosphere containing 5% CO2. The number of viable cells at the end of the incubation period is measured using MTS assay. Absorbance at 490nm is read and subtracted with non-specific absorbance measured at 630nm. Wells containing medium without cells serve as blanks. Cell viability is calculated as a percentage compared to the control cells, which are arbitrarily assigned 100% viability. The half maximal inhibitory concentration (IC50) values are graphically obtained from the dose-response curves |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
