| 中文名称: | Eukaryotic Initiation Factor | ||||
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| 英文名称: | Eukaryotic Initiation Factor | ||||
| CAS No: | 432499-63-3 | 分子式: | C22H14ClNO4S2 | 分子量: | 455.93 |
| CAS No: | 432499-63-3 | ||||
| 分子式: | C22H14ClNO4S2 | ||||
| 分子量: | 455.93 | ||||
基本信息
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产品编号： |
E10373 |
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产品名称： |
Eukaryotic Initiation Factor |
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CAS: |
432499-63-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
455.93 |
-20℃ |
一个月 |
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化学名： |
5-(5-((3-Benzyl-4-oxo-2-thioxothiazolidin-5-ylidene)methyl)furan-2-yl)-2-chlorobenzoic acid |
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Solubility (25°C)： |
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体外:
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DMSO |
20mg/mL(43.8664mM;Need ultrasonic) |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:2.33mg/mL(5.11mM);Suspended solution; Need ultrasonic |
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此⽅案可获得2.33mg/mL(5.11mM)的均匀悬浊液，悬浊液可⽤于⼝服和腹腔注射。以1mL⼯作液为例，取100μL23.3mg/mL的澄清DMSO储备液加到400μL PEG300中，混合均匀；向上述体系中加⼊50μL Tween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.1933mL |
10.9666mL |
21.9332mL |
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5mM |
0.4387mL |
2.1933mL |
4.3866mL |
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10mM |
0.2193mL |
1.0967mL |
2.1933mL |
生物活性
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产品描述 |
是eIF4E-eIF4G相互作用的抑制剂，IC50值为13.5μM。 |
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靶点 |
IC50: 13.5μM (eIF4E-eIF4G) |
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体外研究 |
4E2RCat prevents the interaction between eIF4E (the cap-binding protein) and eIF4G (a large scaffolding protein), inhibiting cap-dependent translation. It significantly decreases human coronavirus 229E (HCoV-229E) replication, reducing the percentage of infected cells and intra- and extracellular infectious virus titers. 4E2RCat inhibits cap-dependent translation in a dose-dependent manner. 4E2RCat inhibits cap-dependent FF translation but not EMCV IRES-driven Ren translation. 4E2RCat inhibits coronavirus replication in a dose- and time-dependent manner |
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体内研究 |
4E2RCat inhibits protein synthesis in vivo and it is not a consequence of increased cell death |
推荐实验方法(仅供参考)
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Cell Assay |
L132 cells are treated with 12.5μM 4E2RCat for the indicated times and are processed for annexin V/propidium iodide staining. To this end, cell medium is collected. Cells are ished with 1 mL PBS, which is collected as well, and trypsinized in 200μL 0.05% trypsin-EDTA. Cells are pooled with previously collected supernatants and spun for 2 min at 2,000 rpm and 4°C. The cell pellet is ished with 2mL cold PBS, followed by another spin. After the second spin, the cell pellet is resuspended in 100μL annexin V binding buffer and propidium iodide added to a final concentration of 5μg/mL. After the addition of 5μL annexin V-fluorescein isothiocyanate, samples are incubated for 15 min in the dark at room temperature and diluted. Fluorescence-activated cell sorter (FACS) analyses are performed using a FACScan instrument |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
