基本信息
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产品编号： |
E10091 |
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产品名称： |
Ethyl gallate |
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CAS: |
831-61-8 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
198.17 |
-20℃ |
1个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
39mg/mL (196.8mM) |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(12.62mM);Clear solution |
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此⽅案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液。 以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μ L Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL。 |
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2.请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(12.62mM);Clear solution |
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此⽅案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液。 以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均 匀。 |
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3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(12.62mM);Clear solution |
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此⽅案可获得 ≥ 2.5 mg/mL (12.62 mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
5.0462mL |
25.2309mL |
50.4617mL |
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5mM |
1.0092mL |
5.0462mL |
10.0923mL |
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10mM |
0.5046mL |
2.5231mL |
5.0462mL |
生物活性
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产品描述 |
Ethyl gallate是一种非类黄酮酚化合物，也是过氧化氢的清除剂。 |
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靶点 |
Bacterial |
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体外研究 |
Ethyl gallate is a nonflavonoid phenolic compound and also a scavenger of hydrogen peroxide.After treatment for 24h or 48h with Ethyl gallate,HL-60 cells show changes in morphology,including shrinkage of the cell membrane and the development of apoptotic bodies.Consistent with these effects,the viability of Ethyl gallate-treated cells decreases in a time- and dose-dependent manner,demonstrating that Ethyl gallate has a cytotoxic effect on HL-60 cells.Ethyl gallate treatment increases the proportion of cells in subG1 phase in a concentration- and time-dependent manner.Treatment of cells for 24h or 48h with 50μM or 75μM Ethyl gallate increases the percentage of cells in the subG1 phase from a baseline of 2.9% to 26.5% or 52.6%,respectively.It is found that Ethyl gallate treatment of HL-60 cells decreases the expression of Bcl-2 at 75μM Ethyl gallate,and increases Bax and truncated Bid (tBid) expression at 24h. |
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体内研究 |
No significant difference in the serum total protein,albumin,globulin and glucose is found between the rats fed with A.nilotica (L.) leaf extract on ethyl gallate equivalent basis and those fed with Ethyl gallate alone.Significant differences in total bilirubin level,however,exist between the rats that receive A.nilotica (L.) leaf extract,500mg/kg body weight (ethyl gallate equivalent of 10mg/kg,0.34±0.01mg/dL) and those receiving 10mg/kg body weight of Ethyl gallate (0.26±0.01mg/dL).Significant difference is found for ALT between groups fed with 500 and 1000mg/kg body weight of A.nilotica (L.)leaf extract (26.52±1.23 and 30.05±1.38 U/L) and 10 and 20mg/kg of Ethyl gallate (20.50±0.94 and 24.67±1.13 U/L). |
推荐实验方法(仅供参考)
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激酶实验： |
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The expression of apoptosis-related proteins (caspases-8,-9,-3;AIF;Endo G;Bid;Bax;and Bcl-2) in HL-60 cells is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of lysates followed by western blotting. For this,HL-60 cells (1.5×106) are treated with 50μM or 75μM Ethyl gallate for 6h,12h,or 24h.Total cell lysates are obtained by resuspending cells in ice-cold radioimmunoprecipitation assay (RIPA) buffer for 30 min followed by centrifugation.Protein concentration is determined using a NanoDrop spectrophotometer.Aliquots of lysates (100μg protein equivalents) are resolved by 12% SDS-PAGE and transferred onto nitrocellulose membranes. |
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细胞实验: |
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HL-60 cells (1×106) are treated with 50μM or 75μM Ethyl gallate for 24h or 48 h at 37℃. Cells are then harvested by centrifugation and fixed in 70% ethanol at 4℃ for 24h.Fixed cells are resuspended in PBS containing 40μg/mL Propidium iodide (PI),100μg/mL RNase A,and 0.1% Triton X-100 and incubated in the dark for 30 min at room temperature.Cell cycle distribution is analyzed by flow cytometry on a FACSCalibur.To investigate apoptotic cells,HL-60 cells (1×106) incubated with different concentration of 50 μM, 75 μM and 100 μM Ethyl gallate for 24 h or 48 h at 37°C, and then DAPI staining is conducted. The cells are photographed using a fluorescence microscopy. |
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动物实验: |
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Forty eight female albino Wistar rats of six to eight weeks old are used and divided into eight groups based on their body weights.Group 1 rats serve as control receiving 1.0mL of the vehicle (0.1% ethanol);Group 2 rats receive A.nilotica (L.) leaf extract (250mg/kg body weight);Group 3 rats receive A.nilotica (L.) leaf extract (500mg/kg body weight);Group 4 rats receive A.nilotica (L.) leaf extract (1000mg/kg body weight);Group 5 rats receive A.nilotica (L.) leaf extract (2000mg/kg body weight);Group 6 rats receive Ethyl gallate (5mg/kg body weight);Group 7 rats receive Ethyl gallate (10mg/kg body weight); Group 8 rats receive Ethyl gallate (20mg/kg body weight).Body weights are recorded on 0th and 14th day for each group and all rats are decapitated after an overnight fast. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
