基本信息
产品编号: |
E10074 |
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产品名称: |
Emodin |
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CAS: |
518-82-1 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
270.24 |
-20℃ |
一个月 |
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化学名: |
1,3,8-trihydroxy-6-methylanthracene-9,10-dione |
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Solubility (25°C): |
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体外:
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DMSO |
54mg/mL (199.82mM) |
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Ethanol |
3mg/mL (11.1mM) |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂:0.5% MC→0.5% Tween-80 Solubility: 10mg/mL (37.00mM); Suspended solution; Need ultrasonic |
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2.请依序添加每种溶剂:0.5% CMC-Na/saline water Solubility: 3.33mg/mL (12.32mM); Suspened solution; Need ultrasonic |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.7004mL |
18.5021mL |
37.0041mL |
5mM |
0.7401mL |
3.7004mL |
7.4008mL |
10mM |
0.3700mL |
1.8502mL |
3.7004mL |
50mM |
0.0740mL |
0.3700mL |
0.7401mL |
生物活性
产品描述 |
抑制NF-κB活化和粘附分子表达。酪蛋白激酶2(CK2)抑制剂。 |
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靶点 |
11β-HSD1 |
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体外研究 |
Emodin (10-400μM) blocks the binding of S protein to ACE2 in a dose-dependent manner with the IC50 value of 200μM. Emodin (5-50μM) inhibits the S protein-pseudotyped retrovirus infectivity in a dose-dependent manner. Emodin blocks the SARS-CoV S protein binding to Vero E6 cells. Emodin inhibits casein kinase-2 (CK2) with IC50s of 5.9, 30.0, and 7.1μM for CK2α Wild-type, Ile174Ala mutant, and His160Ala mutant at ATP concentration is 50μM, respectively. The IC50s are 1.40 and 38.00μM for CK2α Wild-type, and Val66Ala mutant at ATP concentration is 10μM. Emodin exhibits low inhibitory activity against mouse and human 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), with an IC50 higher than 1mM, indicating that Emodin is more than 5000-fold selective for the human and mouse 11β-HSD1 enzymes over the type 2 isoenzyme Cell Viability Assay |
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Cell Line: |
Vero E6 cells transfected with the plasmid encoding ACE2 |
Concentration: |
0, 5, 25, 50μM |
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Incubation Time: |
24 hours |
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Result: |
Vero cells treated with 50μM remained 82.4±3.8% viability, the anti-SARS-CoV activity was not due to toxicity. |
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体内研究 |
Emodin (single oral administration of 100 or 200mg/kg) inhibits 11β-HSD1 activity in normal C57BL/6J male mice. Emodin (100mg/kg; oral administration; b.i.d.) improves insulin sensitivity and lipid metabolism, and lowers blood glucose and hepatic PEPCK, and glucose-6-phosphatase mRNA in diet-induced obese (DIO) mice |
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Animal Model: |
C57BL/6J male mice |
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Dosage: |
100 or 200mg/kg |
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Administration: |
Acute administered p.o. ; Two hours later, the mice were killed by cervical dislocation, |
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Result: |
Significantly inhibited liver 11β-HSD1 enzymatic activity by 17.6 and 31.3% and mesenteric fat 11β-HSD1 enzymatic activity by 21.5 and 46.7% at 100 or 200mg/kg, respectively. |
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Animal Model: |
DIO mice (C57BL/6J male mice were fed a formulated research diet) |
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Dosage: |
100mg/kg |
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Administration: |
Oral gavage; twice per day; for 35 days |
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Result: |
Reduced fasting glucose concentrations to 77.2% of the vehicle control mice after 7 days of treatment, and these remained significantly lower throughout the treatment period. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )