中文名称: 骨化三醇 促销
英文名称: 1α,25-Dihydroxyvitamin D3
CAS No: 32222-06-3
分子式: C27H44O3
分子量: 416.64
EINEC: 250-963-8
D60002 骨化三醇 ≥99%(HPLC) (psaitong)
外观与性状:
白色或类白色粉末
包装规格:
5mg 25mg 100mg in glass bottle
溶解性:
溶于乙醇(83mg/ml)、二甲基亚砜(83MG/ML)、甲醇和氯仿
产品描述:

基本信息

产品编号:

D60002

产品名称:

1α,25-Dihydroxyvitamin D3

CAS:

32222-06-3

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C27H44O3

溶于液体

 

 

分子量

416.64

 

 

化学名: 

1A,25-dihydroxycholecalciferol

Solubility (25°C):

 

体外:

 

DMSO

83 mg/mL (199.21mM)

Ethanol

83 mg/mL (199.21mM)

Water

Insoluble

体内(现配现用):

1.请依序添加每种溶剂: 10% DMSO40% PEG3005% Tween-8045% saline

Solubility: 2.75 mg/mL (6.60mM); Suspended solution; Need ultrasonic

此方案可获得 2.75 mg/mL (6.60mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

1mL 工作液为例,取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加入50μL Tween-80,混合均匀;然后继续加入 450μL生理盐水定容至 1mL。

2.请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution

此方案可获得 ≥ 2.75 mg/mL (6.60mM,饱和度未知) 的澄清溶液。

1mL 工作液为例,取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

3.请依序添加每种溶剂: 10% DMSO90% corn oil

Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution

此方案可获得 ≥ 2.75 mg/mL (6.60mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

1mL 工作液为例,取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900μL玉米油中,混合均匀。

4.请依序添加每种溶剂: 5% DMSO40% PEG3005% Tween-8050% saline

Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution

5.请依序添加每种溶剂: 5% DMSO95% (20% SBE-β-CD in saline)

Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution

6.请依序添加每种溶剂: 10% EtOH40% PEG3005% Tween-8045% saline

Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.00mM,饱和度未知) 的澄清溶液。

1mL 工作液为例,取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加入50μL Tween-80,混合均匀;然后继续加入 450μL生理盐水定容至 1mL。

7.请依序添加每种溶剂: 10% EtOH90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (6.00mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.00mM,饱和度未知) 的澄清溶液。

1mL 工作液为例,取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

8.请依序添加每种溶剂: 10% EtOH90% corn oil

Solubility: ≥ 2.5 mg/mL (6.00mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.00mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

1mL 工作液为例,取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900μL玉米油中,混合均匀。

9.请依序添加每种溶剂: 1% DMSO99% saline

Solubility: 0.55 mg/mL (1.32mM); Suspended solution; Need ultrasonic

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

 1mM

2.4002mL

12.0008mL

24.0015mL

5mM

0.4800mL

2.4002mL

4.8003mL

10mM

0.2400mL

1.2001mL

2.4002mL

50mM

0.0480mL

0.2400mL

0.4800mL

 

生物活性

产品描述

一种PKC和VDR的激活剂。

靶点

vitamin D receptor 
(Cell-free assay)

体外研究

Calcitriol exerts antiproliferative effects on cervical cancer cells in vitro. Cells decrease by 12.8% when treated with 100 nM Calcitriol for 6 days, compare with control. Inhibition of cell proliferation becomes more pronounced with the increase in Calcitriol concentration. The decrease is 26.1% and 31.6% for 200 and 500nM Calcitriol, respectively. Treatment with Calcitriol for 72 h induces an evident accumulation of cells in the G1 phase, with approximately 66.18% in 200nM and 78.10% in 500nM, compare with the control (24.36%). Calcitriol treatment significantly decreases HCCR-1 protein expression compare with the control in a time- and dose-dependent manner. Calcitriol significantly increases ERα mRNA in a dose dependent manner with an EC50 of 9.8×10-9 M.

体内研究

Chronic treatment with Calcitriol (150 ng/kg per day for 4.5 months) improves the relaxations (pD2: 6.30±0.09, Emax: 68.6±3.9% in Calcitriol-treated OVX, n=8). Renal blood flow in OVX rats is reduced in both kidneys, and the flow is restored by Calcitriol treatment. The increased expression of COX-2 and Thromboxane-prostanoid (TP) receptor in OVX rat renal arteries is reduced by chronic calcitriol administration. High- and low-dose Calcitriol treatment significantly decreases the systolic blood pressure (SBP) in the fructose-fed rats by 14±4 and 9±4mmHg, respectively, at Day 56. High-dose Calcitriol treatment (20ng/kg per day) significantly increases serum ionized calcium level (1.44±0.05 mmol/L) compare with the other groups.

特征

 

 

推荐实验方法(仅供参考)

Cell Assay

HeLa S3 cells are plated at a density of 1,000 cells/well in 96-well plates of Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), treated with 1% ethanol (control) or various concentrations of Calcitriol (100, 200, and 500nM) for 72 h. A Cell Counting Kit8 (CCK-8) is used to determine cell proliferation. At 24, 48, 72, 96, 120, and 144 h after culturing with 200nM Calcitriol, cells are harvested for analysis. Three independent experiments are performed in quadruplicate

 

Animal Administration

Adult female Sprague-Dawley rats weighing 200 to 220g are used in this study. Rats are housed in a temperature-controlled room (~23°C) with a 12-h light/dark cycle. The animals have free access to a standard diet and water. Ovariectomy (OVX) is performed on rats. At 6 months after the surgical procedure, the OVX rats are randomly assigned to either treatment with vehicle dimethyl sulfoxide (OVX+vehicle) or Calcitriol (150ng/kg daily, OVX+calcitriol). Calcitriol treatment is given by oral gavage and lasted or 4.5 months. Blood pressure and serum Calcitriol level are measured.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):