| 中文名称: | 盐酸多西环素 促销 | ||||
|---|---|---|---|---|---|
| 英文名称: | Doxycycline HCl | ||||
| 别名: | 盐酸强力环素 Doxycycline hydrochloride | ||||
| CAS No: | 10592-13-9 | 分子式: | C22H24N2O8.HCl | 分子量: | 480.9 |
| CAS No: | 10592-13-9 | ||||
| 分子式: | C22H24N2O8.HCl | ||||
| 分子量: | 480.9 | ||||
| EINEC: | 234-198-7 | ||||
| EINEC: | 234-198-7 | ||||
包装规格：
1g 5g in glass bottle
产品简介：
Doxycycline hydrochloride 是一种具有口服活性的四环素抗生素，是广谱的金属蛋白酶 (MMP) 抑制剂，具有抗菌活性和抗癌细胞增殖活性。
溶解性：
溶于水(50mg/mL)
储备液保存：
-80°C, 6 months; -20°C, 1 month
体外研究：
Doxycycline hydrochloride (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations.
Doxycycline hydrochloride (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells.
Doxycycline hydrochloride (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines.
Doxycycline hydrochloride (0-250 μM, 72 h) inhibits cell viability of breast cancer cells .
Doxycycline hydrochloride (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells.
Doxycycline hydrochloride (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines.
Doxycycline hydrochloride (0-250 μM, 72 h) inhibits cell viability of breast cancer cells .
体内研究：
Doxycycline hydrochloride (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner.
激酶实验：
Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size.
细胞实验：
Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-H3]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA.
注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。
2、以上信息仅做参考交流之用。
2、以上信息仅做参考交流之用。
保存条件：
-20℃
UN码：
HazardClass：
危害声明：
安全说明：
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