Didox - CAS:69839-83-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Didox
英文名称:	Didox
别名:	3,4-二羟基苯甲羟肟酸 NSC-324360
CAS No:	69839-83-4	分子式:	C₇H₇NO₄	分子量:	169.13
CAS No:	69839-83-4
分子式:	C₇H₇NO₄
分子量:	169.13
EINEC:	7909
EINEC:	7909

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

D10804 Didox ≥98% (psaitong)

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包装规格：

10mg 50mg 100mg in glass bottle

溶解性：

溶于DMSO（100 mg/mL 超声）

产品描述：

基本信息

产品编号：D10804
产品名称：Didox
CAS:	69839-83-4	储存条件	粉末	-20℃	四年

分子式：	C7H7NO4		溶于液体	-80℃	六个月
分子量	169.13			-20℃	一个月
化学名：	3,4-Dihydroxybenzohydroxamic acid
Solubility (25°C)	体外	DMSO	100mg/mL (591.26mM; Need ultrasonic)
		Ethanol
		Water
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	5.9126mL	29.5631mL	59.1261mL
5mM	1.1825mL	5.9126mL	11.8252mL
10mM	0.5913mL	2.9563mL	5.9126mL

生物活性

产品描述	一种合成的核糖核苷酸还原酶 (ribonucleotide reductase, RR) 抑制剂。
靶点/IC₅₀	Ribonucleotide reductase
体外研究	Didox (NSC-324360) suppresses LPS-induced mRNA levels of iNOS, IL-6, IL-1, TNF-α, NF-κβ (p65), and p38-α, after 24 h of treatment. Treatment with Didox also suppresses the secretion of nitric oxide (NO), IL-6, and IL-10. Using mitochondrial dehydrogenase activity as a measure of cytotoxicity, the effects of Didox on cellular respiration in RAW264.7 are examined over a range of concentrations for 24h. Cells exposures to 200μM and below Didox, with and without LPS, do not exhibit significant cellular toxicity. Didox (NSC-324360) is active against all human and murine acute myeloid leukemia (AML) lines tested with IC50 values in the low micromolar range (mean IC50 37 µM [range 25.89-52.70 µM]).
体内研究	Once engraftment is established by bioluminescent imaging, the animals receive daily administrations of Didox at 425mg/kg via IP injection over 5 days. Didox (NSC-324360) treatment significantly reduces leukemic burden compared to vehicle treated controls (p=0.0026 and p=0.0342). More importantly, Didox (NSC-324360) provides a significant survival benefit(p<0.0001 and p=0.0094).

推荐实验方法(仅供参考)

细胞实验：

Cell Assay

RAW264.7 macrophages are treated with Didox alone, with 0.1μg/mL LPS, or the two in combination. Cellular respiration, as an indication of cytotoxicity, is measured by the MTT assay, which quantifies mitochondrial dehydrogenase activity. Macrophages are plated into 96 well Costar plates at 105 cells per well in 100μL of DMEM media. After 4h of incubation at 37°C for adherence, compounds and DMSO carrier control (0.01% final) are added in triplicate over serial dilutions beginning with 200μM per well in a total volume of 200μL, and the plates incubated for 24 h. Four h before termination of the assay, each well receives 20μL of a 5 mg/mL MTT solution in un-supplemented DMEM. After centrifugation, the supernatant for each well is discarded and cells containing reduced MTT are solubilized with 100μL of acidified isopropanol(4 mM HCl, 0.1% NP-40 in isopropanol). Following a brief period of shaking, the optical density (O.D.) for each well is recorded at 550nm. Each experiment is repeated three times and the data averaged from each triplicate, then expressed as percentage of the control O.D. values for each experiment

动物实验:

Animal Administration

Mice

Luciferase-tagged leukemia cells are transplanted into 8- week old, sublethally irradiated (4.5Gy) C57Bl/6 mice by tail vein injection of 1.0×106 cells per mouse. Mice are injected with 150mg/kg D-Luciferin, anesthetised with Isoflurane, and imaged using the IVIS 100 imaging system. Mice begin treatment with Didox upon detection of clear signal. The animals are treated with daily administrations of Didox (NSC-324360) at 425mg/kg Didox by intraperitoneal injection (IP) for 5 days. Control animals receive 5% dextrose water by IP injection. Repeat imaging is performed on the day following the final treatment.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果