| 中文名称: | DMU-212 | ||||
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| 英文名称: | DMU-212 | ||||
| 别名: | 1,2,3-三甲氧基-5-[(1e)-2-(4-甲氧基苯基)乙烯]苯;3,4,5,4''-四甲氧基二苯乙烯;3,4,5,4'-四甲氧基二苯乙烯;3,4,5,4-四甲氧基二苯乙烯 (E)-3,4,5,4′-Tetramethoxystilbene;MR-4 | ||||
| CAS No: | 134029-62-2 | 分子式: | C18H20O4 | 分子量: | 300.35 |
| CAS No: | 134029-62-2 | ||||
| 分子式: | C18H20O4 | ||||
| 分子量: | 300.35 | ||||
基本信息
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产品编号： |
D10778 |
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产品名称： |
DMU-212 |
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CAS: |
134029-62-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
300.35 |
-20℃ |
一个月 |
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化学名： |
(E)-3,4,5,4′-Tetramethoxystilbene |
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Solubility (25°C)： |
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体外:
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DMSO |
20mg/mL(66.59mM;Need ultrasonic) |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2mg/mL(6.66mM);Clear solution |
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此⽅案可获得≥2mg/mL(6.66mM，饱和度未知)的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL20.0mg/mL的澄清DMSO储备液加到900μL⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.3294mL |
16.6472mL |
33.2945mL |
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5mM |
0.6659mL |
3.3294mL |
6.6589mL |
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10mM |
0.3329mL |
1.6647mL |
3.3294mL |
生物活性
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产品描述 |
一种白藜芦醇甲基化衍生物，具有抗分裂、抗增殖、抗氧化和促进细胞凋亡的活性。 |
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体外研究 |
DMU-212 (0.3125-40μM) inhibits growth of A375, MeWo, Bro and M5 human melanoma cells DMU-212 (30-50μM; 24 hours) induces upregulation of cell cycle inhibitors, apoptosis and ERK activation in A375 cells Cell Proliferation Assay |
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Cell Line: |
A375 cells, MeWo cells, M5 cells, Bro cells |
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Concentration: |
0.3125μM, 0,625μM, 1.25μM, 2.5μM, 5μM, 10μM, 20μM, 40μM |
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Incubation Time: |
96 hours |
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Result: |
Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5μM for A375 and Bro and IC50= 1.25μM for MeWo and M5 cells). |
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Cell Cycle Analysis |
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Cell Line: |
A375 cells |
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Concentration: |
20μM, 30μM, 50μM |
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Incubation Time: |
24 hours |
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Result: |
Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2. |
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Western Blot Analysis |
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Cell Line: |
A375 cells |
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Concentration: |
20μM, 30μM, 50μM |
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Incubation Time: |
24 hours |
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Result: |
Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2. |
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Apoptosis Analysis |
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Cell Line: |
A375 cells |
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Concentration: |
10μM, 20μM |
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Incubation Time: |
24 hours, 36 hours |
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Result: |
Induced apoptosis. |
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体内研究 |
DMU-212 (50mg/kg; i.g.; three times a week; for 14 days) inhibits tumor growth in xenograft model of human ovarian cancer |
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Animal Model: |
6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts |
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Dosage: |
50mg/kg |
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Administration: |
Oral gavage, three times a week, for 14 days |
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Result: |
Lowered tumor burden. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
