| 中文名称: | DPBQ | ||||
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| 英文名称: | DPBQ | ||||
| 别名: | 2,3-Diphenylbenzo[g]quinoxaline-5,10-dione | ||||
| CAS No: | 7029-89-2 | 分子式: | C24H14N2O2 | 分子量: | 362.38 |
| CAS No: | 7029-89-2 | ||||
| 分子式: | C24H14N2O2 | ||||
| 分子量: | 362.38 | ||||
基本信息
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产品编号：D10762 |
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产品名称：DPBQ |
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CAS: |
7029-89-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
362.38 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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生物活性
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产品描述 |
一种有效的凋亡诱导剂，对高倍体细胞具有特异性。DPBQ诱导p53的表达和磷酸化，这种作用是四倍体细胞特有的。 |
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靶点/IC50 |
p53 |
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体外研究 |
DPBQ elicits apoptosis and cell death and is selective for effects in 4N cells. DPBQ elicits expression and phosphorylation of p53 and this effect is specific to tetraploid cells. Knockdown of TP53 restores proliferation of tetraploid cells in the presence of DPBQ.For Western blot analysis, 2N and 4N RPE1 cells are treated with DMSO,50ng/mL doxorubicin, or 1μM DPBQ for 48 hours.Western blotting is performed with a SDS-PAGE Electrophoresis and semidry transfer. Samples are analyzed bychemiluminescence. β-actin is used as a protein loading control. |
推荐实验方法(仅供参考)
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激酶实验： |
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For Western blot analysis, 2N and 4N RPE1 cells are treated with DMSO, 50ng/mL doxorubicin, or 1μM DPBQ for 48 hours.Western blotting is performed with a SDS-PAGE Electrophoresis and semidry transfer. Samples are analyzed bychemiluminescence.β-actin is used as a protein loading control. |
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细胞实验: |
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For Apoptosis assays, 2N and 4N RPE1 cells are treated with DMSO,60nM staurosporine, or 1μM DPBQ for 48 hours.Apoptosis is evaluated by an Annexin V-PE/7-AAD apoptosis detection kit. For the flow cytometry analysis, the percent positive cells in the upper right and lower right quadrants are summed to give the total number of apoptotic cells. Three independent replicates are performed. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
