基本信息
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产品编号： |
D10151 |
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产品名称： |
1,8-Dihydroxyanthraquinone |
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CAS: |
117-10-2 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
240.21 |
-20℃ |
一个月 |
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化学名： |
1,8-dihydroxy-9,10-dihydroanthracene-9,10-dione |
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Solubility (25°C)： |
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体外:
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DMSO |
5mg/mL(20.82mM;ultrasonic and warming and heat to 60°C) |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
4.1630mL |
20.8151mL |
41.6302mL |
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5mM |
0.8326mL |
4.1630mL |
8.3260mL |
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10mM |
0.4163mL |
2.0815mL |
4.1630mL |
生物活性
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产品描述 |
Danthron 是⼀种从中药 rhubarb 中提取的天然产物。Danthron 通过激活 AMPK 来调节葡萄糖和脂质代谢。 |
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靶点 |
AMPK |
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体外研究 |
Danthron (0.1, 1, and 10μM) dose-dependently promotes the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in both HepG2 and C2C12 cells. Meanwhile, Danthron treatment significantly reduces the lipid synthesis related sterol regulatory element-binding protein 1c (SREBP1c) and fatty acid synthetase (FAS) gene expressions, and the total cholesterol (TC) and triglyceride (TG) levels. In addition, Danthron treatment efficiently increases glucose consumption. Danthron effectively reduces intracellular lipid contents and enhances glucose consumption in vitro via activation of AMPK signaling pathway. 10μM Danthron/24 h is safe for HepG2 cells. With 80% confluence, HepG2 cells are incubated with Danthron (0.1- 10μM) in FBS-Free media for 8 h. Subsequently, cells are harvested for Western blot assay. Danthron increases the p-AMPK protein in a dose-dependent manner, and no changes in t-AMPK protein are observed. Danthron inhibits 9-cis retinoic acid (9cRA)-induced retinoic X receptor α (RXRα) transactivation by IC50 at 0.11μM. To further clarify the stoichimetric ratio of Danthron binding to RXRα-ligand-binding domain (LBD), isothermal titration calorimetry (ITC) experiment is performed. The KD value of Danthron binds to RXRα-LBD by ITC experiment is determined at 7.5μM |
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体内研究 |
Danthron functions as an insulin sensitizer in vivo. Danthron improves insulin sensitivity in diet-induced obese (DIO) mice. The insulin tolerance test result shows that Danthron (5mg/kg) treated diet-induced obesity mice exhibit lower glucose levels after insulin challenge, compared with the control vehicle-treated group |
推荐实验方法(仅供参考)
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Cell Assay |
HepG2 cells are transfected with pGL3-ABCA1 promoter-luc or pGL3-ABCG1 promoter-luc and pRL-SV40 plasmids. At 6 h post-transfection, the cells are incubated with Danthron (0-20μM), TO90 (2μM) or DMSO for 24 h. Luciferase activity is measured using the Dual Luciferase Reporter Assay kit |
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Animal Administration |
Mice C57/BL6 male mice are fed with a high fat diet for 3 months and treated with Danthron (5mg/kg) or vehicle orally for 8 weeks. The animals are then fasted for 6 h and then given intraperitoneal injection of insulin at 1.5 units/kg. Blood samples are analyzed at 15, 30, 45, 60, 90, and 120 min using Accu-Chek active blood sugar test meter. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
