基本信息
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产品编号： |
D10147 |
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产品名称： |
Daidzein |
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CAS: |
486-66-8 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
254.24 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
51mg/mL(200.59mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(9.83mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(9.83mM，饱和度未知)的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到900μL⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.9333mL |
19.6665mL |
39.3329mL |
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5mM |
0.7867mL |
3.9333mL |
7.8666mL |
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10mM |
0.3933mL |
1.9666mL |
3.9333mL |
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50mM |
0.0787mL |
0.3933mL |
0.7867mL |
生物活性
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产品描述 |
一种植物雌激素。 |
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靶点 |
PPAR-α |
PPAR-γ |
Human Endogenous Metabolite |
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体外研究 |
Daidzein以剂量依赖的方式抑制iNOS蛋白和mRNA表达以及NO的产生。Daidzein还抑制信号转导和转录1（STAT-1）的活化剂的活化，STAT-1是iNOS的另一个重要的转录因子。在细胞中，Daidzein（1μM和10μM）引起蛋白质含量，碱性磷酸酶活性以及DNA含量的显著增加，分别达1.4-，1.5和2.0倍。Daidzein（2-50mM）增加成骨细胞的生存能力约1.4倍。Daidzein（2-100mM）增加碱性磷酸酶活性和成骨细胞中骨钙素合成分别约1.4-和2.0倍。Daidzein刺激不同阶段成骨细胞的分化（从成骨前体细胞终末分化成骨细胞）。Daidzein (1μM and 10μM) causes a significant increase of alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in bone tissues. Daidzein-induced increase of calcium content and alkaline phosphatase activity in bone tissues is completely prevented by cycloheximide (1μM). |
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体内研究 |
在雌性大鼠中，Daidzein减少体重，这可以通过减少饲料消耗进行解释。在雌性大鼠中，Daidzein导致轻微但不显著的卵巢和子宫的重量以及乳腺大小降低。 |
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推荐实验方法(仅供参考)
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Cell Assay |
HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid |
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Animal Administration |
Mice Experiments are performed in 10- to 11-week-old male C57 (C57 bl/6) and Apoe KO (C57 background) mice. For long-term stroke recovery, mice receive Moxifloxacin (100mg/kg) for 3 d. The prophylactic antibiotic treatment is shown to effectively reduce mortality in an animal model of stroke by attenuating peripheral infection. In addition, saline is subcutaneously administered daily, and hydrogel (Clear H2O) is given to prevent dehydration. With the implementation of poststroke care (antibiotic regimen, rehydration, and feeding hydrogels with soft diet) during the acute period ( |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
