多西他赛 - CAS:114977-28-5 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	多西他赛促销
英文名称:	Docetaxel
别名:	多烯紫杉醇 Docetaxel
CAS No:	114977-28-5	分子式:	C₄₃H₅₃NO₁₄	分子量:	807.88
CAS No:	114977-28-5
分子式:	C₄₃H₅₃NO₁₄
分子量:	807.88
MDL:	MFCD00800737	EINEC:	628503
EINEC:	628503

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

D10131 多西他赛 ≥98%(HPLC),分析标准品 (psaitong)

相关产品

包装规格：

20mg in glass bottle

溶解性：

熔点：186-192°C 溶于DMSO (25mg/ml)和乙醇(25mg/ml)

产品描述：

基本信息

产品编号：D10131
产品名称：Docetaxel
CAS:	114977-28-5	储存条件	粉末	-20℃	四年
分子式：	C43H53NO14		溶于液体	-80℃(避光)	六个月
分子量：	807.88			-20℃(避光)	一个月
化学名：
Solubility (25°C)	体外	DMSO	100mg/mL (123.78mM)
		Ethanol	100mg/mL warmed with 50ºC water bath (123.78mM)
		Water	Insoluble
	体内（现配现用）	5% DMSO+30% PEG 300+5% Tween 80+ddH2O	2mg/mL
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	1.2378mL	6.1890mL	12.3781mL
5mM	0.2476mL	1.2378mL	2.4756mL
10mM	0.1238mL	0.6189mL	1.2378mL
50mM	0.0248mL	0.1238mL	0.2476mL

生物活性

产品描述	一种紫杉类抗癌药物，它结合并稳定微管蛋白亚基，有丝分裂纺锤体的解聚，从而导致细胞周期阻滞和凋亡。
靶点/IC50	Microtubules (Cell-free assay)
体外研究	Docetaxel (RP-56976) and Glufosfamide (GLU) single and combined treatments affect the cells viability in a dose-dependent manner. The IC50 of GLU are 70±4µM and 86.8±8µM in PC-3 and LNCaP cells; respectively. While, the IC50 of Docetaxel alone is found to be 3.08±0.4nM and 1.46±0.2nM in PC-3 and LNCaP cells;respectively.The co-treatment of GLU with Docetaxel is found to synergize the cytotoxicity and the IC50 values are decreased to be 2.7±0.1nM and 0.75±0.3nM in PC-3 and LNCaP cells; respectively.IC50 of NCI-H460 to Docetaxel at 24 h is 116nM and at 72 h is 30nM. According to data reported in DTP Data Search, the mean IC50 of NCI-60 cell panel to Docetaxel is 14-34nM
体内研究	In female mice, the Docetaxel (RP-56976)-induced intestinal apoptosis in the 14-hours after light on (HALO) group is significantly greater than that in the 2-HALO group. Bax expression is significantly elevated by Docetaxel in the 2-HALO group, but not in the 14-HALO group. On the other hand, cleaved Caspase-3 expression is significantly elevated by Docetaxel in the 14-HALO group, but not in the 2-HALO group. The expressions of Wee1 and phosphorylated CKD1 are significantly elevated after dosing of Docetaxel at 14 HALO, but not at 2 HALO. In addition,Docetaxel significantly reduces survivin expression in the 14-HALO group but not in the 2-HALO group. The survivin expression level in the Docetaxel-treated 14-HALO group is significantly smaller than that in the drug-treated 2-HALO group.Piperine (PIP) is administrated via intravenous bolus at 3.5mg/kg and via oral administration at 35mg/kg and 3.5mg/kg,while Docetaxel (DOX) is intravenously administrated at 7mg/kg to Sprague-Daley rats. The co administrations of PIP at 35mg/kg via oral administration and Docetaxel at 7mg/kg via intravenous bolus administration in Sprague-Dawley rats. The combination use of PIP and Docetaxel results in a synergic increase of both their in vivo exposure.

推荐实验方法(仅供参考)

细胞实验:

Single-drug concentration-response curves are assessed. Seeding is done at a density of 2,000 cells/well for PC-3 and LNCaP, in 96-well plates. Cells are treated with each single drug and their combination for 72h at different drug concentrations. Docetaxel is used at concentrations of 0.1-1,000nM. GLU is used at concentrations of 0.1-300µm.Cytotoxicity is assessed at the end of drug exposure using SRB assay. Following 72 h exposure the cells are fixed with 10% trichloroacetic acid (150µL) for 1 h at 4℃. Then, cells are stained for 10 min at room temperature with 0.4% SRB dissolved in 1% acetic acid. The plates are then air dried for 24h and the dye is made soluble with 150µL Tris (10mM, PH 7.4) for 5 min on a shaker at 1,600 rpm. Absorbance is then measured at 545nM using microplate reader. Results are expressed as the relative percentage of absorbance compared to control.

动物实验:

Mice

Five-week-old male Balb/c mice are used. Docetaxel (0, 10, 20, 30, 40, 60, and 80mg/kg per week) is given once a week for 3 weeks for mice. Because more than 30mg/kg per week of Docetaxel causes body weight loss in mice, 20mg/kg per week of Docetaxel is judged to be the maximum nontoxic dose. Docetaxel (20mg/kg per week) is given to mice once a week for 3 weeks at one of the following different points (2, 10, 14, or 22 HALO). Seventy-two hours after the final dosing of the agent,the intestinal mucosa of the small intestine (proximal 8cm) is removed, fixed in 20 N Mildform solution (containing 8% formaldehyde in a buffered solution), and embedded in paraffin blocks, and sections of 5μm are put on glass slides.Apoptosis is detected using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) method,using the Apop Tag Peroxidase In Situ Apoptosis Detection Kit.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果