姜黄素 - CAS:458-37-7 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	姜黄素促销
英文名称:	Curcumin
别名:	川芎内酯 Curcumin
CAS No:	458-37-7	分子式:	C₂₁H₂₀O₆	分子量:	368.38
CAS No:	458-37-7
分子式:	C₂₁H₂₀O₆
分子量:	368.38
MDL:	MFCD00008365	EINEC:	207-280-5
EINEC:	207-280-5

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C70006 姜黄素 ≥65% (psaitong)

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熔点：

183°C

外观与性状：

橙黄色结晶性粉末

包装规格：

5g 25g 100g in glass bottle

产品描述：

基本信息

产品编号：	C70006
产品名称：	Curcumin
CAS:	458-37-7	储存条件	粉末	-20℃	四年

分子式：	C21H20O6		溶于液体	-80℃	两年
分子量	368.38			-20℃	1个月
化学名：	(1E,6E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione
Solubility (25°C)：
体外:	DMSO	73 mg/mL (198.16mM)
	Water	Insoluble
	Ethanol	Insoluble
体内(现配现用):	1.请依序添加每种溶剂： 1% (w/v) carboxymethylcellulose (CMC) Solubility: 25 mg/mL (67.86mM); Suspension solution; Need ultrasonic
	2.请依序添加每种溶剂： 10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 3 mg/mL (8.14mM); Clear solution
	此方案可获得 ≥ 3 mg/mL (8.14mM，饱和度未知) 的澄清溶液。以 1mL 工作液为例，取 100μL 30.0 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加入50μL Tween-80，混合均匀；然后继续加入 450μL生理盐水定容至 1mL。
	3.请依序添加每种溶剂： 10% DMSO→90% (20% SBE-β-CD in saline) Solubility: 3 mg/mL (8.14mM); Suspended solution; Need ultrasonic
	此方案可获得 3 mg/mL (8.14mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。以 1mL 工作液为例，取 100μL 30.0 mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.7146mL	13.5729mL	27.1459mL
5mM	0.5429mL	2.7146mL	5.4292mL
10mM	0.2715mL	1.3573mL	2.7146mL

生物活性

产品描述	制造姜黄试纸，pH 8～9。测定硼。
靶点	Keap1-Nrf2, Histone acetyltransferase
体外研究	Curcumin exerts its chemopreventive effects partly through the activation of nuclear factor (erythroid-2 related) factor 2 (Nrf2) and its antioxidant and phase II detoxifying enzymes. Curcumin inhibits T47D cells growth, with IC50s of 25, 19 and 17.5μM for 24, 48 and 72 h MTT assays respectively. IC50s of curcumin and silibinin mixture against T47D cells, are 17.5, 15, and 12μM for 24, 48, and 72 h exposure times, respectively. Curcumin (2.5-80μM) induces apoptotic cell death in AGS and HT-29 cell lines, and the IC50 is 21.9±0.1, 40.7±0.5μM, respectively, in both AGS and HT-29 cell lines. Curcumin-induced apoptosis requires caspase activities in AGS and HT-29 cells. Curcumin induces ER Ca2+ decline and mitochondrial Ca2+ overloading. Curcumin induces the G2/M cell cycle arrest of LNCaP and PC-3 cells in a dose dependent manner. Curcumin upregulates the protein level of NF-kappaB inhibitor IkappaBalpha and downregulates protein levels of c-Jun and AR[5].
体内研究	Curcumin (10 mg/kg, p.o.) significantly prevents decrease in the percentage of sucrose consumption, as compared to the CMS-exposed rats. Curcumin treatment results in significant prevention of increase in TNF-α and IL-6 levels in stressed rats[4]. Curcumin decreases binding of p300/CREB-binding protein (CBP) at the brain-derived neurotrophic factor (BDNF) promoter at 20 mg/kg (i.p.), reduces binding of P300/CBP at the BDNF promoter at 40 mg/kg, and decreases binding all the four proteins of p300/CBP and H3K9ac/H4K5ac at the BDNF promoter at 60 mg/kg in chronic constriction injury (CCI) rats

推荐实验方法(仅供参考)

Cell Assay

T47D breast cancer cell line is grown in RPMI 1640 supplemented with 10% FBS, 2 mg/mL sodium bicarbonate, 0.05 mg/mL penicillin G, 0.08 mg/mL streptomycin. Culture is maintained on plastic flask and incubated at 37°C in 5% CO2. After growing sufficient amount of cells, cytotoxic effect of silibinin and curcumin is studied by 24, 48 and 72 h MTT assays in which 1000 cell/well are cultivated in a 96 well plate. After 24 h incubation in 37°C with humidified atmosphere containing 5% CO2, the cells are treated with serial concentrations of curcumin (5, 10, 20, 30, 40, 50, 60, 80, 100 µM), silibinin (20, 40, 60, 80, 100, 120, 140, 180, 200 µM), and curcumin-silibinin mixture (each of them 5, 10, 20, 30, 40, 50, 60, 80, 100 µM) for 24, 48 and 72 h in the quadruplicate manner, in addition to cells with 200μL culture medium containing 10% DMSO for control. After incubation, the medium of all wells of the plate are exchanged with fresh medium and the cells are leaved for 24 h in incubator. Then, medium of all wells are removed carefully and 50μL of 2 mg/mL MTT dissolved in PBS is added to each wells and the plate is covered with aluminum foil and incubated for 4.5 h again. After removing content of the wells, 200μL pure DMSO is added to the wells. Then, 25μL Sorensen’s glycine buffer is added and immediately absorbance of each wells is read in 570nm using EL×800 Microplate Absorbance Reader with reference wavelength of 630nm.

Animal Administration

Curcumin (10 mg/kg), freshly suspended in saline, is administrated by oral gavage once a day for 3 weeks. Forty rats are randomLy assigned to 4 groups (n=10/each group): group I receives saline and serves as control, group II receives curcumin, group III is exposed to CMS andreceive saline and group IV are subjected to CMS andreceive curcumin.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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