基本信息
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产品编号： |
C60010 |
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产品名称： |
Crotaline |
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CAS: |
315-22-0 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
325.36 |
-20℃ |
一个月 |
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化学名： |
(13-alpha,14-alpha)-14,19-Dihydro-12,13-dihydroxy-20-norcrotalanan-11,15-dione |
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Solubility (25°C)： |
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体外:
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DMSO |
65mg/mL (199.77mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂：20% HP-β-CD in saline Solubility: 21mg/mL (64.54mM); Clear solution; Need ultrasonic and warming and heat to 53°C |
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2.请依序添加每种溶剂：5% DMSO→40% PEG300→5% Tween-80→50% saline Solubility: ≥ 2.5mg/mL (7.68mM); Clear solution |
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3.请依序添加每种溶剂：5% DMSO→95% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (7.68mM); Suspended solution |
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4.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (6.39mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (6.39mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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5.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08mg/mL (6.39mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (6.39mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 |
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6.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (6.39mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (6.39mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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7.请依序添加每种溶剂：1% DMSO→99% saline Solubility: ≥ 0.5mg/mL (1.54mM); Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.0735mL |
15.3676mL |
30.7352mL |
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5mM |
0.6147mL |
3.0735mL |
6.1470mL |
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10mM |
0.3074mL |
1.5368mL |
3.0735mL |
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50mM |
0.0615mL |
0.3074mL |
0.6147mL |
生物活性
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产品描述 |
一种吡咯烷啶生物碱,用于诱导大鼠肺部疾病. |
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体外研究 |
Monocrotaline (MCT) is an 11-membered macrocyclic pyrrolizidine alkaloid (PA) derived from the seeds of the Crotalaria spectabilis plant. Monocrotaline a natural ligand exhibits dose-dependent cytotoxicity with potent antineoplastic activity. The in vitro cytotoxicity of monocrotaline is proved at IC50 24.966 µg/mL and genotoxicity at 2 X IC50 against HepG2 cells |
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体内研究 |
MCT causes a pulmonary vascular syndrome in rats characterized by proliferative pulmonary vasculitis, pulmonary hypertension (PH), and cor pulmonale. Among preclinical models of pulmonary arterial hypertension (PAH), monocrotaline animal model offers the advantage of mimic several key aspects of human PAH, including vascular remodeling, proliferation of smooth muscle cells, endothelial dysfunction, upregulation of inflammatory cytokines, and right ventricle failure, requiring a single drug injection. Changes in multiple pathways associated with the development of PH, including activated glycolysis, increased markers of proliferation, disruptions in carnitine homeostasis, increased inflammatory and fibrosis biomarkers, and a reduction in glutathione biosynthesis are observed with the injection of monocrotaline |
推荐实验方法(仅供参考)
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Animal Administration |
Rats: A total of 20 male Sprague Dawley rats (SD; 220-270g) are used in this study (n=10 per group). Control group received vehicle for monocrotaline (MCT). Pre-pulmonary hypertension (PH) group received a single injection of MCT (60mg/kg i.p.) to induce and are sacrificed after 14 days |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
