| 中文名称: | CRF, bovine | ||||
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| 英文名称: | CRF, bovine | ||||
| 别名: | Corticotropin Releasing Factor bovine;Ser-Gln-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Thr-Lys-Ala-Asp-Gln-Leu-Ala-Gln-Gln-Ala-His-Asn-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2 | ||||
| CAS No: | 92307-52-3 | 分子式: | C206H340N60O63S | 分子量: | 4697.34 |
| CAS No: | 92307-52-3 | ||||
| 分子式: | C206H340N60O63S | ||||
| 分子量: | 4697.34 | ||||
基本信息
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产品编号： |
C111014 |
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产品名称： |
CRF,bovine |
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CAS: |
92307-52-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
二年 |
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分子量： |
4697.34 |
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化学名： |
Ser-Gln-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Thr-Lys-Ala-Asp-Gln-Leu-Ala-Gln-Gln-Ala-His-Asn-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2 |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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生物活性
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产品描述 |
一种有效的 CRF 受体激动剂，能够取代 [125I-Tyr]ovine CRF，Ki 值为3.52nM。 |
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靶点 |
Ki:3.52nM (CRF receptor) |
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体外研究 |
CRF,bovine is a potent agonist of CRF receptor,and displaces [125I-Tyr]ovine CRF with a Ki of 3.52nM.CRF shows pEC50s of 11.16,8.53 and 8.70 for human CRF1,human CRF2 and rat CRF2α.CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress,and leads to production of glucocorticoids which down regulate immune responses.CRF also has proinflammatory effects.CRF affects brain microvessel endothelial cells (BMEC) structure or function,CRF (100nM) significantly increases cAMP in BMEC. |
推荐实验方法(仅供参考)
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细胞实验: |
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CRF (1μM) is added to the cell cultures that are further incubated for 5,15 and 30 min at 37℃.Control cells are incubated with medium only.Following incubation time, cells are lysed directly on the growth dish using the detergent provided by the cAMP enzyme immunoassay kit.Following trypan blue staining to ensure complete lysis,the cell lysate is collected and assayed for cAMP.In some cases,the brain microvessel endothelial cells (BMEC) are treated with forskolin or pretreated either with the CRFR antagonist Antalarmin (1μM) or the ATP analogue 2′5′-deoxyadenosine for 5 min at 37℃. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
