| 中文名称: | Ca2+ channel agonist 1 促销 | ||||
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| 英文名称: | Ca2+ channel agonist 1 | ||||
| 别名: | Ca2+ channel agonist 1 Ca2+ channel agonist 1 | ||||
| CAS No: | 1402821-24-2 | 分子式: | C19H26N6O | 分子量: | 354.45 |
| CAS No: | 1402821-24-2 | ||||
| 分子式: | C19H26N6O | ||||
| 分子量: | 354.45 | ||||
基本信息
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产品编号： |
C10909 |
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产品名称： |
Ca2+ channel agonist 1 |
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CAS: |
1402821-24-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
354.45 |
-20℃ |
1个月 |
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化学名： |
(R)-2-(6-(Benzylamino)-9-propyl-9H-purin-2-ylamino)butan-1-ol |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(7.05mM);Clear solution |
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此⽅案可获得 ≥ 2.5 mg/mL (7.05 mM，饱和度未知) 的澄清溶液。 以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μ L Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL |
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2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(7.05mM);Clear solution |
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此⽅案可获得 ≥ 2.5 mg/mL (7.05 mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1 mL ⼯作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.8213mL |
14.1064mL |
28.2127mL |
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5mM |
0.5643mL |
2.8213mL |
5.6425mL |
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10mM |
0.2821mL |
1.4106mL |
2.8213mL |
生物活性
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产品描述 |
一种N-型钙离子通道(N-type Ca2+ channel)激动剂和Cdk2的抑制剂，EC50分别为14.23μM和3.34μM，对运动神经终端功能障碍有潜在疗效。 |
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靶点 |
CDK2 3.34μM (EC50) |
Type Ca2+ Channel 14.23μM (EC50) |
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体外研究 |
Ca2+ channel agonist 1 (Compound 13d) is an agonist of N-type Ca2+ channel and an inhibitor of Cdk2,with EC50s of 14.23μM and 3.34μM,respectively.Ca2+ channel agonist 1 exhibits a ca.2-fold increased agonism and a 22-fold decreased cdk2 kinase activity versus the standard,(R)-roscovitine. |
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推荐实验方法(仅供参考)
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细胞实验: |
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Briefly,the pipet solution consists of 70nM Cs2SO4,60mM CsCl,1mM MgCl2,and 10mM HEPES at pH 7.4.Cultured cells are bathed in a saline composed of 130mM choline chloride (ChCl),10mM tetraethylammonium chloride (TEA-Cl),2mM CaCl2,1mM MgCl2,and 10mM HEPES at pH 7.4. Patch pipettes are fabricated from borosilicate glass,and capacitive currents and passive membrane responses to voltage commands are subtracted.Currents are amplified by an amplifier,filtered at 5 kHz,and digitized at 10 kHz for subsequent analysis.A liquid junction potential of −11.3 mV is subtracted during recordings.To measure effects on calcium channel tail currents,the tail current integral is measured before and after application of a derivative (including Ca2+ channel agonist 1),with the integral of each trace being normalized to its peak. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
