| 中文名称: | Cynaropicrin | ||||
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| 英文名称: | Cynaropicrin | ||||
| 别名: | 菜蓟苦素 [(3aR,4S,6aR,8S,9aR,9bR)-8-hydroxy-3,6,9-trimethylidene-2-oxo-3a,4,5,6a,7,8,9a,9b-octahydroazuleno[4,5-b]furan-4-yl] 2-(hydroxymethyl)prop-2-enoate | ||||
| CAS No: | 35730-78-0 | 分子式: | C19H22O6 | 分子量: | 346.37 |
| CAS No: | 35730-78-0 | ||||
| 分子式: | C19H22O6 | ||||
| 分子量: | 346.37 | ||||
基本信息
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产品编号： |
C10867 |
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产品名称： |
Cynaropicrin |
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CAS: |
35730-78-0 |
储存条件 |
粉末 |
-20℃(避光) |
四年 |
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分子式： |
溶于液体 |
-80℃(避光) |
6个月 |
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分子量： |
346.37 |
-20℃(避光) |
1个月 |
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化学名： |
[(3aR,4S,6aR,8S,9aR,9bR)-8-hydroxy-3,6,9-trimethylidene-2-oxo-3a,4,5,6a,7,8,9a,9b-octahydroazuleno[4,5-b]furan-4-yl] 2-(hydroxymethyl)prop-2-enoate |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.8871mL |
14.4354mL |
28.8709mL |
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5mM |
0.5774mL |
2.8871mL |
5.7742mL |
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10mM |
0.2887mL |
1.4435mL |
2.8871mL |
生物活性
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产品描述 |
一种倍半萜内酯，可以抑制肿瘤坏死因子(TNF-α)的释放，在鼠和人巨噬细胞的IC50值分别为8.24和3.18μM。 |
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靶点 |
MMP;NF-κB;TNF Receptor |
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体外研究 |
Cynaropicrin strongly inhibits lipopolysaccharide-induced TNF-α release from either murine or human macrophage cells in a dose-dependent manner with the IC50 values of 8.24 and 3.18μM,respectively.Cynaropicrin shows significant inhibitory effects toward all mitogenic signals with the IC50 values of 1.20 (concanavalin A),1.02 (phytohemagglutinin) and 0.90μM (lipopolysaccharide),respectively. Cynaropicrin suppresses CTLL-2 cell proliferation in a dose-dependent manner and the 50% inhibitory concentration (IC50) of Cynaropicrin for CTLL-2 cell growth is 0.91μM.The increased mRNA expression of MMP13 induced by TNF-α is similarly inhibited in a concentration-dependent manner by Cynaropicrin.The increased mRNA expression of HIF-2α induced by IL-1β in SW1353 is inhibited in a concentration-dependent manner by Cynaropicrin. |
推荐实验方法(仅供参考)
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细胞实验: |
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Human U937 cells are cultured in RPMI1640 supplemented with 10% fetal bovine serum.To differentiate U937 cells,2×106 cells/mL are treated with phorbol 12-myristate 13-acetate (PMA) of 20ng/mL for 24h.The PMA is removed by washing and adherent cells are then allowed to recuperate for 40h.The recuperated cells are subsequently incubated with lipopolysaccharide of 1μg/mL for 6h with Cynaropicrin and positive control drugs.Supernatants are harvested and assayed by ELISA kit for human TNF-α. |
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动物实验: |
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Male Swiss mice are used in this study.Mice are housed at a maximum of 8 per cage and kept in a conventional room at 20 to 24℃ under a 12h to 12h light-dark cycle.The animals are provided with sterilized water and chow ad libitum.Infection is performed by i.p.injection of 104 or 5×103 bloodstream trypomastigotes.The animals (18 to 21g) are divided into the following groups (at least five mice per group):uninfected (noninfected and untreated),untreated (infected with T.cruzi but treated only with vehicle),and treated (infected and treated i.p.with 0.5 to 50mg/kg/day compound (including Cynaropicrin) or 100mg/kg/day benznidazole).Mice receive 0.1mL (i.p.) at 5 and 8 days postinfection (dpi),or at 11,12,and 13 dpi for the dose of 25mg/kg,twice a day (b.i.d.). |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
