| 中文名称: | 柯因 | ||||
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| 英文名称: | Chrysin | ||||
| 别名: | 5,7-二羟黄酮 白杨素 Chrysin;5,7-Dihydroxyflavone;Chrysin-5,7-dihydroxy Flavone;5,7-dihydroxy-2-phenyl-4H-benzo(b)pyran-4-one;5,7-Dihydroxy-2-phenyl-4H-chromen-4-one;5,7-dihydroxy-2-phenylchromen-4-one;CHRYSIN(AS);CHRYSIN(AS) PrintBack;5,7-Dihydroxy-2-phenyl-4H-benzo[b]pyran-4-one;5;[ "5", "7-Dihydroxyflavone" ] | ||||
| CAS No: | 480-40-0 | 分子式: | C15H10O4 | 分子量: | 254.24 |
| CAS No: | 480-40-0 | ||||
| 分子式: | C15H10O4 | ||||
| 分子量: | 254.24 | ||||
| MDL: | MFCD00006834 | EINEC: | 207-549-7 | ||
| EINEC: | 207-549-7 | ||||
基本信息
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产品编号： |
C10135 |
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产品名称： |
Chrysin |
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CAS: |
480-40-0 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
254.24 |
-20℃ |
一个月 |
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化学名： |
5,7-dihydroxy-2-phenyl-4H-benzo(b)pyran-4-one |
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Solubility (25°C)： |
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体外:
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DMSO |
51mg/mL (200.59mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (9.83mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (9.83mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (9.83mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (9.83mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.9333mL |
19.6665mL |
39.3329mL |
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5mM |
0.7867mL |
3.9333mL |
7.8666mL |
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10mM |
0.3933mL |
1.9666mL |
3.9333mL |
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50mM |
0.0787mL |
0.3933mL |
0.7867mL |
生物活性
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产品描述 |
一种黄酮类化合物，类似于芹菜素。它抑制芳香化酶，也抑制潜伏感染模型中HIV的激活。素具有抗炎和抗氧化作用。诱导各种细胞凋亡，从而表现出抗癌的化学预防活性。 |
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靶点 |
estrogen |
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体外研究 |
Chrysin is mainly found in passion flowers, honey, and propolis acts as a potential therapeutic and preventive agent to inhibit proliferation and invasion of various human cancer cells. Although Chrysin has anti-carcinogenic effects in several cancers, little is known about its functional roles in ovarian cancer which shows poor prognosis and chemoresistance to traditional therapeutic agents. Chrysin inhibits ovarian cancer cell proliferation and induced cell death by increasing reactive oxygen species (ROS) production and cytoplasmic Ca2+ levels as well as inducing loss of mitochondrial membrane potential (MMP). Chrysin activates MAPK and PI3K/AKT pathways in ES2 and OV90 cells in concentration-response experiments. Chrysin suppresses tumor growth byregulating canonical Wnt and nuclear factor NF-κB signaling cascades cancer cells. Chrysin stimulates the phosphorylation of AKT and P70S6K proteins in both ES2 and OV90 cells compared tothe untreated control cells. In addition, Chrysin activates the phospho-ERK1/2, p38,and JNK proteins as members of the MAPK pathway in the ovarian cancer cells |
推荐实验方法(仅供参考)
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Cell Assay |
The proliferation assays are conducted using a cell proliferation enzyme-linked immunosorbent assay (ELISA) 5-bromo-2'- deoxyuridine (BrdU) kit. Briefly, ES2 and OV90 cells are seeded in a 96-well plate, and then treated with Chrysin (0, 5, 10, 20, 50, and 100μM) with or without inhibitors (20μM LY294002, PI3K/AKT; 10μM U0126, ERK1/2; 10μM SP600125, JNK; and 20μM SB203580, p38) in a final volume of 100μL/well. Aftera48-h incubation, 10μM BrdU is added to the cell culture, followed by an additional 2-h incubation at 37°C. After labeling the cells with BrdU, they are fixed and then incubated with the antiBrdU-peroxidase (POD) working solution for 90 min. The anti-BrdU-POD binds to the BrdU incorporated into newly synthesized cellular DNA and these immune complexes are detected by analyzing their reaction with the 3,3',5,5'- tetramethylbenzidine (TMB) substrate. The absorbance values of the reaction product are measured at 370 and 492 nm using an ELISA reader |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
