羰基氰酯-3-氯苯基腙 - CAS:555-60-2 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	羰基氰酯-3-氯苯基腙
英文名称:	Carbonyl cyanide 3-chlorophenylhydrazone
别名:	CCCP Mesoxalonitrile 3-chlorophenylhydrazone CCCP Mesoxalonitrile 3-chlorophenylhydrazone
CAS No:	555-60-2	分子式:	C₉H₅ClN₄	分子量:	204.62
CAS No:	555-60-2
分子式:	C₉H₅ClN₄
分子量:	204.62
MDL:	MFCD00001848	EINEC:	209-103-7
EINEC:	209-103-7

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

C10089 羰基氰酯-3-氯苯基腙 ≥99% (psaitong)

相关产品

半胆碱-3
CAS No：312-45-8
1,2-双(2-氨基苯氧基)乙烷-N,N,N',N'-四乙酸四乙酯
CAS No：73630-07-6
BAPTA 四钾盐
CAS No：73630-08-7
BAPTA 四甲酯
CAS No：125367-34-2
吩嗪硫酸乙酯
CAS No：10510-77-7
亚精胺
CAS No：124-20-9

包装规格：

100mg 500mg in glass bottle

溶解性：

溶于DMSO(20mg/ml)

产品描述：

基本信息

产品编号：	C10089
产品名称：	Carbonyl cyanide 3-chlorophenylhydrazone
CAS:	555-60-2	储存条件	粉末	-20℃	四年

分子式：	C9H5ClN4		溶于液体	-80℃	两年
分子量	204.62			-20℃	一个月
化学名：	2-[(3-Chlorophenyl)hydrazinylidene]propanedinitrile
Solubility (25°C)：
体外:	DMSO	41mg/mL (200.37mM)
	Ethanol	21mg/mL (102.62mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (12.22mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (12.22mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	4.8871mL	24.4355mL	48.8711mL
5mM	0.9774mL	4.8871mL	9.7742mL
10mM	0.4887mL	2.4436mL	4.8871mL
50mM	0.0977mL	0.4887mL	0.9774mL

生物活性

产品描述	一种线粒体中氧化磷酸化的原核团（H+ ionophore）和解偶联子，显示对胞内钙有多种作用。亦抑制肝脏脂肪酶的分泌。
靶点	STING IFN-β
体外研究	CCCP inhibits IFN-β production induced by various types of the STING pathway activators. CCCP suppresses the phosphorylation of STING, TBK1, and IRF3 via disrupting the association of STING and TBK1. CCCP inhibits activation of STING and its downstream signaling molecules, TBK1 and IRF3, but not STING translocation to the perinuclear region. CCCP impairs the interaction between STING and TBK1 and concomitantly triggers mitochondria fission. Importantly, the knockout of the crucial mitochondria fission regulator Drp1 restored the STING activity, indicating that CCCP downmodulates the STING pathway through DRP1-mediated mitochondria fragmentation. The protonophore CCCP that disrupts membrane potential suppresses the DMXAA-triggered STING signaling pathway. CCCP drastically suppresses the production of IFN-β in DMXAA-treated RAW264.7 cells and MEFs
体内研究	The same dosage of 3mg/kg.bw each of CCCP and PPEF is used. In both the cases 1 log reduction is observed in the bacterial load. However, when 3mg/kg.bw of PPEF is used in combination with 3mg/kg.bw of CCCP, 6 log10 reduction is observed in the bacterial count. The developed model validates the enhanced antibacterial activity of combination therapy[2] . ^99mTcMIBI signals in the hearts of SD rats administered CCCP (4mg/kg intraperitoneally) or vehicle is also measured. ^99mTc-MIBI signals decrease in rat hearts administered CCCP, and the ATP content, as measured by ³¹P magnetic resonance spectroscopy, decreased simultaneously. To investigate whether CCCP decreased the ^99mTc-MIBI signals in rats, we analyzed the radioisotope activity of excised heart tissue from rats administered CCCP. At 180 min after ^99mTc-MIBI injection, the ^99mTc-MIBI signals from the hearts in the CCCP group are significantly lower than those in the vehicle group

推荐实验方法(仅供参考)

Cell Assay

MEFs (5×10⁵ ), Raw264.7 cells (1×10⁶ ), and HeLa cells stable expressing STING (1.5×10⁵ ) are stimulated with DMXAA (100 μ g/mL) for 2 or 3 h, or transfected with c-di-GMP (5μM), cGAMP (5μg/mL), or poly (dA:dT) (2μg/mL) for 6 h. CCCP (50μM) is co-treated with DMXAA (100μg/mL), or treated for the last 5 h in case of treatment of c-di-GMP or poly (dA:dT)

Animal Administration

Mice

Female Balb/c mice n=6, per dosing group weighing 20-25 g are rendered neutropenic with 2 intraperitoneal injections of cyclophosphamide 150mg/kg.bw and 100mg/kg.bw on 4 days and 1 day prior to bacterial infection. 0.1mL of the 10⁶ CFU/mL bacterial suspension is injected into right posterior thigh muscle. After 2 h post-infection mice are treated with PPEF (3mg/kg.bw), CCCP (3mg/kg.bw) and in combination PPEF+CCCP (3mg/kg.bw+3mg/kg.bw) dissolved in 0.1mL sterile water by single bolus intravenous injection. Twenty-four hours after antibacterial administration, the mice are humanely sacrificed. Right thigh muscles from each mouse are aseptically collected, homogenized and serially diluted and processed for quantitative cultures.

Rats

Rats are randomly divided into three groups. One group is euthanized 15 min after a dose of 12.5 MBq (337.8 μCi) ^99mTc-MIBI injection (n=6). The other two groups are administered 4mg/kg CCCP (CCCP group; n=7) or vehicle (vehicle group; n=7) by intraperitoneal (i.p.) injection 90 min after the same dose of ^99mTc-MIBI injection and are euthanized after an additional 90 min (180 min after the ^99mTc-MIBI injection). Hearts are excised and weighed, and radioactivity is measured between 110 and 170 keV with an auto-well gamma counter. ^99mTc-MIBI signals are corrected for physical decay (half-life=6 h).

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

点击下载核磁/氢谱图谱(PDF)

搜索质检报告(COA)

参考文献 & 客户发表文献