中文名称: | 甲酚紫 | ||||
---|---|---|---|---|---|
英文名称: | Cresyl Violet acetate | ||||
别名: | 焦油紫 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate;(9-aminobenzo[a]phenoxazin-5-ylidene)azanium;acetate | ||||
CAS No: | 10510-54-0 | 分子式: | C18H15N3O3 | 分子量: | 321.33 |
CAS No: | 10510-54-0 | ||||
分子式: | C18H15N3O3 | ||||
分子量: | 321.33 | ||||
MDL: | MFCD00013151 |
基本信息
产品编号: |
C10077 |
||||
产品名称: |
Cresyl Violet acetate |
||||
CAS: |
10510-54-0 |
储存条件 |
粉末 |
室温 |
四年 |
|
|
||||
分子式: |
溶于液体 |
-80℃ |
6个月 |
||
分子量 |
321.33 |
-20℃ |
1个月 |
||
化学名: |
3,7-Diaminobenzo[b]phenoxazinylium acetate |
||||
Solubility (25°C): |
|||||
体外:
|
DMSO |
1 mg/mL (3.11mM; Need ultrasonic) |
|||
Water |
0.67 mg/mL (2.09mM; Need ultrasonic) |
||||
Ethanol |
|
||||
体内(现配现用): |
|
||||
<1mg/ml表示微溶或不溶。 |
|||||
普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
|||||
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.1121mL |
15.5603mL |
31.1207mL |
生物活性
产品描述 |
用于脑石蜡切片和脊髓切片的尼氏染色。 |
体外研究 |
The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. |
推荐实验方法(仅供参考)
Cell Assay |
Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl violet acetate and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl violet acetate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl violet acetate staining sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )