| 中文名称: | Bikinin 促销 | ||||
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| 英文名称: | Bikinin | ||||
| 别名: | 4-((5-Bromo-2-pyridinyl)amino)-4-oxobutanoic acid 4-[(5-Bromo-2-pyridinyl)amino]-4-oxo-butanoic acid Abrasin | ||||
| CAS No: | 188011-69-0 | 分子式: | C9H9BrN2O3 | 分子量: | 273.08 |
| CAS No: | 188011-69-0 | ||||
| 分子式: | C9H9BrN2O3 | ||||
| 分子量: | 273.08 | ||||
| MDL: | MFCD00116442 | ||||
基本信息
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产品编号： |
B70022 |
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产品名称： |
Bikinin |
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CAS: |
188011-69-0 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
273.08 |
-20℃ |
1个月 |
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化学名： |
4-((5-Bromo-2-pyridinyl)amino)-4-oxobutanoic acid 4-[(5-Bromo-2-pyridinyl)amino]-4-oxo-butanoic acid Abrasin |
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Solubility (25°C)： |
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体外:
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DMSO |
54mg/mL (197.74mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶 1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(9.15mM);Clear solution 2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(9.15mM);Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.6619mL |
18.3097mL |
36.6193mL |
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5mM |
0.7324mL |
3.6619mL |
7.3239mL |
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10mM |
0.3662mL |
1.8310mL |
3.6619mL |
生物活性
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产品描述 |
一种Arabidopsis GSK-3抑制剂，作为油菜素内酯(BR)信号的一种强效活化剂发挥作用。 |
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靶点 |
GSK-3 |
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体外研究 |
Bikinin reduces transcript levels of NbRBOHB and BR-induced NbRBOHB expression in TRV:00,TRV:NbDWARF,TRV:NbBRI1,TRV:NbBSK1,TRV:NbBAK1,TRV:NbBIK1 and TRV:NbBSU1 plants.Bikinin largely inhibits BR-increased ROS accumulation.Moreover,Bikinin decreases the transcripts of these six genes in all plants,but fails to down-regulate transcripts of these genes in NbBES1/BZR1-silenced plants.Bikinin also inhabits BR-induced up-regulation of the six genes,and the inhibition effects are compromised in NbBES1/BZR1-silenced plants.Bikinin is a potent inhibitor of group I and group II ASKs.Bikinin directly binds the GSK3 BIN2 and acts as an ATP competitor.Furthermore,bikinin inhibits the activity of six other Arabidopsis GSK3s. |
推荐实验方法(仅供参考)
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激酶实验： |
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ASKs are expressed as GST-fusion proteins in Escherichia coli BL21.In vitro kinase assays are performed by incubating 50ng GST-fusion protein,10μg myelin basic protein (MBP) as a substrate and 0.15 MBq γ-[32P]-ATP as co-substrate at 25℃ for 30 min.The reaction buffer consists of 20mM HEPES/KOH pH 7.4,15mM MgCl2,5mM EGTA,1mM dithiothreitol and 1μM cold ATP.The reaction products are separated by SDS-PAGE and the amount of radioactivity incorporated into MBP quantified using an Amersham storage phosphor imager screen and a Biorad Molecular Imager FX. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
