| 中文名称: | Benzamil hydrochloride hydrate | ||||
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| 英文名称: | Benzamil hydrochloride hydrate | ||||
| 别名: | N-(Benzylamidino)-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride hydrate | ||||
| CAS No: | 161804-20-2 | 分子式: | C13H14ClN7O.HCl.XH2O | 分子量: | 356.21 |
| CAS No: | 161804-20-2 | ||||
| 分子式: | C13H14ClN7O.HCl.XH2O | ||||
| 分子量: | 356.21 | ||||
基本信息
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产品编号： |
B70010 |
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产品名称： |
Benzamil hydrochloride hydrate |
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CAS: |
161804-20-2 |
储存条件 |
粉末 |
2-8℃ 避光 干燥 |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
356.21 |
-20℃ |
1个月 |
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化学名： |
N-(Benzylamidino)-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride hydrate |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
'mg/mL |
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Water |
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体内(现配现用): |
请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天 使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶。 1.请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% saline Solubility:≥2.5mg/mL(7.02mM);Clear solution 2.请依序添加每种溶剂：10% DMSO 90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(7.02mM);Clear solution 3.请依序添加每种溶剂：10% DMSO 90% corn oil Solubility:≥2.5mg/mL(7.02mM);Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.8073mL |
14.0367mL |
28.0733mL |
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5mM |
0.5615mL |
2.8073mL |
5.6147mL |
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10mM |
0.2807mL |
1.4037mL |
2.8073mL |
生物活性
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产品描述 |
一种钠通道（ENaC）的特异性阻断剂。 |
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靶点 |
Na+/Ca2+Exchanger |
Sodium Channel |
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体外研究 |
Benzamil hydrochloride (Benzylamiloride hydrochloride) inhibits neuronal and heterologously expressed small conductance Ca2+-activated K2+ channels. |
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体内研究 |
Benzamil hydrochloride (Benzylamiloride hydrochloride) (0.7mg/kg/day;s.c.) treated stroke-prone spontaneously hypertensive rats (SHRSP) survived,on average,until 16.1 weeks of age in SHRSP rats. |
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推荐实验方法(仅供参考)
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细胞实验: |
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Individual CCD segments are dissected from rabbit and mouse kidney.The measurements of [Na+]i are used as a reflection of Na+ transport across the apical membrane.EnaC-specific activity is assessed by applying 10−6 M of the ENaC blocker Benzamil hydrochloride,a concentration that does not affect Na+:H+ exchange.Experiments are performed in the presence/absence of luminal Benzamil hydrochloride or the Na:H exchanger blocker HOE694 administers with/without the AT1 receptor blocker candesartan.Final DMSO concentrations are below 0.1%. |
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动物实验: |
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For the acute intracerebroventricular injection in anesthetized rats.A guide cannula is inserted into the right lateral cerebral ventricle,and Benzamil hydrochloride (0.1nmol/kg,n=6;1nmol/kg,n=6;10nmol/kg,n=7) or vehicle (n=6) is injected into the right lateral ventricle through a cannula connected to a microsyringe 15 min before the start of intracerebroventricular infusion of hypertonic NaCl.Each injection consists of a volume of 10μL deliver manually over a period of 30s.To investigate the effects of Benzamil hydrochloride on the pressor response induced by intracerebroventricular injection of the pressor agent other than hypertonic NaCl,endothelin-1,which has a potent pressor action in the brain of rats,is intracerebroventricularly injected (1nmol/10μL) 15 min after the intracerebroventricular preinjection of Benzamil hydrochloride (10nmol/kg,n=6) or the vehicle (n=6),and mean arterial pressure,heart rate,and abdominal sympathetic nerve firings are recorded for 20 min.Catheters are implanted into both the femoral artery and vein of 12-wk-old male rats anesthetized with urethan.Both catheters are filled with heparinized saline (50 U/mL).Fifteen minutes before the start of intracerebroventricular infusion of hypertonic NaCl,Benzamil hydrochloride (10nmol/kg,n=6) or vehicle (10μL,n=6) is injected into a tube connects to the femoral venous catheter by directly inserting the microsyringe,and 0.1ml of isotonic saline solution is injected through the catheter to deliver the contents into the venous circulation. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
