中文名称: | BRL-50481 促销 | ||||
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英文名称: | BRL-50481 | ||||
别名: | 2-甲基-5-硝基-N,N-二甲基苯磺酰胺 N,N,2-Trimethyl-5-nitrobenzenesulfonamide | ||||
CAS No: | 433695-36-4 | 分子式: | C9H12N2O4S | 分子量: | 244.27 |
CAS No: | 433695-36-4 | ||||
分子式: | C9H12N2O4S | ||||
分子量: | 244.27 |
基本信息
产品编号: |
B11404 |
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产品名称: |
BRL-50481 |
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CAS: |
433695-36-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
244.27 |
-20℃ |
一个月 |
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化学名: |
N,N,2-Trimethyl-5-nitrobenzenesulfonamide |
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Solubility (25°C): |
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体外:
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DMSO |
49mg/mL(200.59mM) |
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Ethanol |
49mg/mL(200.59mM) |
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Water |
Insoluble |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
4.0938mL |
20.4692mL |
40.9383mL |
5mM |
0.8188mL |
4.0938mL |
8.1877mL |
10mM |
0.4094mL |
2.0469mL |
4.0938mL |
50mM |
0.0819mL |
0.4094mL |
0.8188mL |
生物活性
产品描述 |
一种PDE7的选择性抑制剂。 |
靶点 |
PDE7 |
体外研究 |
BRL-50481 increases the cAMP content (19.1±6.2% of IBMX response at 300μM) but is considerably less potent. BRL-50481 (30μM) fails to suppress proliferation by itself but significantly potentiates the effect of rolipram. BRL-50481 (30μM) has no effect on IL-15-induced proliferation but augments the inhibitory effect of rolipram. Pretreatment (30 min) of human monocytes with BRL-50481 has, by itself, a negligible (~2 to 10%) inhibitory effect on TNFα output at all concentrations tested. BRL-50481 also potentiates the inhibitory effect of PGE2 on LPS-induced TNFα release. BRL-50481 has no significant effect by itself on κB-dependent transcription (5.6±1.9% inhibition at 30μM) and fails to enhance the effect of rolipram(maximum inhibition, 52.9±2.7%; pIC30 value of 5.33±0.12). BRL-50481 suppresses, in a concentration-dependent manner, LPS-induced TNFα release in monocytes in which PDE7A1 is induced (21.7±1.6% inhibition at 30μM at the 12-h time point) |
推荐实验方法(仅供参考)
Cell Assay |
MOLT-4 cells in 96-well plates are treated for 30 min with BRL-50481 as indicated. The cAMP content is then determined by an immunospecific ELISA. Results are expressed as a percentage of the response affected by 100μM IBMX |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )