中文名称: | BI8626 | ||||
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英文名称: | BI8626 | ||||
别名: | BI8626 N-(3-(Aminomethyl)benzyl)-8-(4-benzylpiperazin-1-yl)pyrimido[5,4-d]pyrimidin-7-amine | ||||
CAS No: | 1875036-75-1 | 分子式: | C25H28N8 | 分子量: | 440.54 |
CAS No: | 1875036-75-1 | ||||
分子式: | C25H28N8 | ||||
分子量: | 440.54 |
基本信息
产品编号: |
B11392 |
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产品名称: |
BI8626 |
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CAS: |
1875036-75-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
440.54 |
-20℃ |
一个月 |
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化学名: |
N-(3-(Aminomethyl)benzyl)-8-(4-benzylpiperazin-1-yl)pyrimido[5,4-d]pyrimidin-7-amine |
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Solubility (25°C): |
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体外:
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DMSO |
80mg/mL(181.5953mM;Need ultrasonic) |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂:10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.08mg/mL(4.72mM);Clear solution |
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此⽅案可获得≥2.08mg/mL(4.72mM,饱和度未知)的澄清溶液。以1mL⼯作液为例,取100μL20.8mg/mL的澄清DMSO储备液加到400μLPEG300中,混合均匀;向上述体系中加⼊50μLTween-80,混合均匀;然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 |
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2.请依序添加每种溶剂:10% DMSO→90% corn oil Solubility:≥2.08mg/mL(4.72mM);Clear solution |
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此⽅案可获得≥2.08mg/mL(4.72mM,饱和度未知)的澄清溶液,此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例,取100μL20.8mg/mL的澄清DMSO储备液加到900μL⽟⽶油中,混合均匀。 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.2699mL |
11.3497mL |
22.6994mL |
5mM |
0.4540mL |
2.2699mL |
4.5399mL |
10mM |
0.2270mL |
1.1350mL |
2.2699mL |
生物活性
产品描述 |
一种泛素连接酶HUWE1特异性拮抗剂。 |
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靶点 |
IC50:0.9μM(HUWE1) |
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体外研究 |
BI8626 induces HUWE1 ectopically expresses to abolishe ubiquitination of MCL1 in HeLa cells. BI8626 suppresses colony formation of Ls174T cells with estimated IC50 value of 0.7μM, and BI8622 (1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. BI8626 (0-50μM; 0-6 hours) retards the degradation of MCL1 in response to UV irradiation to the same extent as depletion of HUWE1 in U2OS cells Cell Cycle Analysis |
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Cell Line: |
Ls174T cells |
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Concentration: |
0μM,5μM,10μM,15μM,20μM |
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Incubation Time: |
0-4 days |
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Result: |
Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. |
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Western Blot Analysis |
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Cell Line: |
U2OS cells |
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Concentration: |
0μM,20μM,50μM |
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Incubation Time: |
0 hour,1 hour,2 hours,4 hours,6 hours |
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Result: |
Retarded the degradation of MCL1 in response to UV irradiation in HeLa cells by inhibiting HUWE1 in U2OS cells. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )