鸡豆黄素A - CAS:491-80-5 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	鸡豆黄素A
英文名称:	Biochanin A
别名:	鹰嘴豆芽素A Biochanin A
CAS No:	491-80-5	分子式:	C₁₆H₁₂O₅	分子量:	284.26
CAS No:	491-80-5
分子式:	C₁₆H₁₂O₅
分子量:	284.26
MDL:	MFCD00006839	EINEC:	207-744-7
EINEC:	207-744-7

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B10129 鸡豆黄素A ≥98%(HPLC) (psaitong)

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包装规格：

200mg in glass bottle

产品描述：

基本信息

产品编号：	B10129
产品名称：	Biochanin A
CAS:	491-80-5	储存条件	粉末	室温	四年

分子式：	C16H12O5		溶于液体	-80℃	两年
分子量	284.26			-20℃	一个月
化学名：	5,7-Dihydroxy-4'-methoxyisoflavone
Solubility (25°C)：
体外:	DMSO	56mg/mL (197.0mM)
	Ethanol	16mg/mL (56.28mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (8.79mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (8.79mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (8.79mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (8.79mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.5179mL	17.5895mL	35.1791mL
5mM	0.7036mL	3.5179mL	7.0358mL
10mM	0.3518mL	1.7590mL	3.5179mL
50mM	0.0704mL	0.3518mL	0.7036mL

生物活性

产品描述	Biochanin A (4-Methylgenistein) 是O-甲基异黄酮，是黄酮类的一种天然有机化合物。
靶点	FAAH (rat) 1.4µM	FAAH (mouse) 1.8µM	FAAH (human) 2.4µM	EGFR 91.5µM
体外研究	Biochanin A被报到能够抑制表皮生长因子受体的蛋白酪氨酸激酶(PTK)，IC50值为91.5µM。Biochanin A抑制小鼠、大鼠和人体内脂肪酸酰胺(FAAH)对0.5µM大麻素的水解作用，IC50值分别为1.8，1.4以及2.4µM。Biochanin A能够抑制血清和EGF刺激的LNCaP和DU-145细胞的生长(对血清的IC50值为8.0到27 微克/毫升，对EGF的IC50值为4.3到15微克/毫升)，但是对EGF受体酪氨酸的自身磷酸化没有显著作用。
体内研究	LD50:小鼠63毫克/千克（腹腔注射)

推荐实验方法(仅供参考)

Kinase Assay

For experiments with FAAH, rat liver homogenates, mouse brain homogenates and membranes from COS7 cells transfected with the human enzyme are used. Frozen (−80°C) livers from adult C57BL/6 mice and frozen brains (minus cerebella) from adult Wistar or Sprague-Dawley rats are thawed and homogenized in 20mM HEPES, 1mMmgCl2, pH 7. The homogenates are centrifuged at ~35000×g for 20 min at 4°C. After resuspension in buffer followed by recentrifugation and a second resuspension in buffer, the pellets are incubated at 37°C for 15 min. This incubation is undertaken in order to hydrolyse all endogenous FAAH substrates. The homogenates are then centrifuged as above, recentrifuged and resuspended in 50mM Tris-HCl buffer, pH 7.4, containing 1mM EDTA and 3mMmgCl2. The homogenates are then frozen at −80°C in aliquots until used for assay. FAAH is assayed in the homogenates and in the COS7 cell membranes using 0.5µM (unless otherwise stated) [ 3H]AEA labelled in the ethanolamine part of the molecule. Blank values are obtained by the use of buffer rather than homogenate. In the experiments comparing effects of Biochanin A upon FAAH and FAAH-2, the same assay is used but with 16 nM [3H]oleoylethanolamide ([3H]OEA) as substrate and with an incubation phase at room temperature. The choice of OEA rather than AEA for FAAH-2 is motivated by the relative rates of hydrolysis: OEA is metabolized four times faster than AEA by FAAH-2, whereas for FAAH the rate of hydrolysis of OEA is about a third of that for AEA. When 0.5µM [3H]AEA is used as substrate, assay conditions for rat brain and mouse liver are chosen so that <10% of added substrate is metabolized. For the human FAAH samples, <5% of the [3H]AEA is metabolized in all cases. For 16 nM [3H]OEA, a limited supply of an expensive ligand meant that optimization is not possible, and the amount of substrate utilized is higher (34±1 and 0.5±0.1% for FAAH and its corresponding mock-transfected, respectively; 40±2 and 21±0.4 for FAAH-2 and its corresponding mocktransfected respectively)

Animal Administration

Mice

ICR mice are used for the behavioural tests measuring spontaneous activity (over a 10 min testing period), rectal temperature, ring immobility (over a 5 min testing period) and nociceptive threshold (tail flick tests). AEA and Biochanin A are dissolved in a vehicle consisting of ethanol, Emulphor-620 and physiological saline in a ratio of 1:1:18 v/v, and administered i.v. to the animals via the tail vein (injection volume 10µL/g body weight). The degree of antinociception is expressed as percentage of maximum possible effect (%MPE), defined as [(test-control time)/(10-control time)]×100.

保存条件：

室温

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果