基本信息
产品编号: |
B10127 |
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产品名称: |
Baicalein |
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CAS: |
491-67-8 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
270.24 |
-20℃ |
一个月 |
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化学名: |
5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one |
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Solubility (25°C): |
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体外:
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DMSO |
54mg/mL (199.82mM) |
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Ethanol |
1mg/mL (3.7mM) |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂:0.5% CMC-Na/saline water Solubility: 20mg/mL (74.01mM); Suspended solution; Need ultrasonic |
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2.请依序添加每种溶剂:10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: 2.5mg/mL (9.25mM); Clear solution; Need ultrasonic |
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此⽅案可获得 2.5mg/mL (9.25mM) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加⼊ 50μL Tween-80,混合均匀;然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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3.请依序添加每种溶剂:10% DMSO→90% (20% SBE-β-CD in saline) Solubility: 2.5mg/mL (9.25mM); Clear solution; Need ultrasonic |
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此⽅案可获得 2.5mg/mL (9.25mM) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中,混合均匀。 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.7004mL |
18.5021mL |
37.0041mL |
5mM |
0.7401mL |
3.7004mL |
7.4008mL |
10mM |
0.3700mL |
1.8502mL |
3.7004mL |
50mM |
0.0740mL |
0.3700mL |
0.7401mL |
生物活性
产品描述 |
Baicalein是CYP2C9和脯氨酰肽链内切酶抑制剂。 |
靶点 |
CYP2C9 |
体外研究 |
Baicalein suppresses mitogen induced T cell proliferation and cytokine secretion in vitro. Pre-treatment with baicalein significantly suppresses Con A or anti-CD3/CD28 mAb induced proliferation as well as cytokine secretion at 25μM. Baicalein treatment induces DNA binding of NF-κB but inhibits thioredoxin activity in the nuclear compartment. Baicalein suppresses proliferation, migration, and invasion of MDA-MB-231 cells in a time- and dose-dependent manner. Baicalein significantly decreases the expression of SATB1 in MDA-MB-231 cells. Baicalein also downregulates the expression of Wnt1 and β-catenin proteins and transcription level of Wnt/β-catenin-targeted genes |
体内研究 |
Baicalein suppresses induction of graft versus host disease but does not inhibit homeostatic proliferation of T-cells in mice. This observation clearly shows potent anti-inflammatory activity of baicalein in vivo. Rats treated with baicalein are protected against an increase in heart to body weight ratio, plasma level of brain natriuretic peptides, intraventricular septum thickness, myocardial collagen volume of left ventricle (all P<0.05, respectively). The antifibrotic effects of baicalein are further illustrated by the suppressed expression of left ventricle pro-collagens I and III accompanied by the decreased expression of 12-lipoxygenase, and by reduced expression and activity of matrix metallopeptidase 9 and extracellular signalregulated kinases. Baicalein can inhibit cardiac fibrosis in hypertensive rats |
推荐实验方法(仅供参考)
Cell Assay |
MTT assay is conducted to evaluate the effect of baicalein on proliferation of breast cancer cells. MDA-MB-231 cells are routinely digested, collected, and then seeded in 96-well plates at a density of 8×103 cells/well. After incubation for 12-24 hours, cells are treated with 0, 20, 40, 60, 80, 100, and 120μM baicalein according to their experimental grouping and then incubated at 37°C for 24, 48, and 72 hours |
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Animal Administration |
Rats: Baicalein is suspended in 1% methylcellulose. Rats are treated with baicalein suspension via oral garvage. SHR and WKY rats are divided into 4 groups (n=8 per group): 12-week treatment with high-dose (200mg/kg/day) or low-dose (50mg/kg/day) group; and 4-week treatment with high-dose or low-dose group. The 12-week and 4-week negative control groups of SHR and WKY rats (n=8 per group) receive vehicle while positive control groups (Val group, n=8 per group) receive valsartan (20mg/kg/day) for comparison. Mice: To study the in vivo anti-inflammatory efficacy of baicalein, graft-versus-host disease (GVHD) model is used. Splenic lymphocytes from C57BL/6 mice are incubated with baicalein in vitro (25μM, 4h) and adoptively transferred to immunecompromised Balb/c mice |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )