盐酸小檗碱 - CAS:633-65-8 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	盐酸小檗碱
英文名称:	Berberine hydrochloride
别名:	盐酸黄连素 9,10-dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium chloride
CAS No:	633-65-8	分子式:	C₂₀H₁₈ClNO₄	分子量:	371.81
CAS No:	633-65-8
分子式:	C₂₀H₁₈ClNO₄
分子量:	371.81
MDL:	MFCD00011939	EINEC:	211-195-9
EINEC:	211-195-9

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

B10055 盐酸小檗碱 ≥98%(HPLC),分析标准品 (psaitong)

相关产品

包装规格：

20mg 100mg in glass bottle

产品描述：

基本信息

产品编号：	B10055
产品名称：	Berberine hydrochloride
CAS:	633-65-8	储存条件	粉末	-20℃	四年

分子式：	C20H18ClNO4		溶于液体	-80℃	两年
分子量	371.81			-20℃	一个月
化学名：	9,10-dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium chloride
Solubility (25°C)：
体外:	DMSO	25mg/mL (67.23mM)
	Ethanol	Insoluble
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：0.5% CMC-Na/saline water Solubility: 11mg/mL (29.59mM); Suspended solution; Need ultrasonic
	2.请依序添加每种溶剂：PBS Solubility: 10mg/mL (26.90mM); Suspended solution; Need ultrasonic
	3.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 1.25mg/mL (3.36mM); Clear solution
	此⽅案可获得 ≥ 1.25mg/mL (3.36mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 12.5mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	4.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 1.25mg/mL (3.36mM); Clear solution
	此⽅案可获得 ≥ 1.25mg/mL (3.36mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 12.5mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.6895mL	13.4477mL	26.8955mL
5mM	0.5379mL	2.6895mL	5.3791mL
10mM	0.2690mL	1.3448mL	2.6895mL
50mM	0.0538mL	0.2690mL	0.5379mL

生物活性

产品描述	一种具有弱抗菌特性的生物碱。
靶点	Caspase-3 ()	Caspase-8 ()		PARP ()	cytochrome c ()	cIAP1 ()
体外研究	Berberine (1.25-160μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29. Berberine (1.25-160μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth. LoVo cells are exposure to Berberine (10-80μM) for 24 h. Cell cycle analysis of 40μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase. Berberine (10-80μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0μM Cell Proliferation Assay
	Cell Line:		Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
	Concentration:		1.25, 2.5, 5, 10, 20, 40, 80, and 160μM
	Incubation Time:		72 hours
	Result:		Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1μM (LoVo) to 98.6±2.9μM (HCT116).
	Cell Proliferation Assay
	Cell Line:		Colorectal carcinoma cell lines LoVo
	Concentration:		1.25, 2.5, 5, 10, 20, 40, 80, and 160μM
	Incubation Time:		24, 48, 72 hours
	Result:		Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0μM induced 71.1±1.9 % growth inhibitions in LoVo cells.
	Cell Cycle Analysis
	Cell Line:		LoVo cells
	Concentration:		0, 10, 20, 40, or 80μM
	Incubation Time:		24 hours
	Result:		Exposure to 40.0μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.
	Western Blot Analysis
	Cell Line:		LoVo cells
	Concentration:		10, 20, 40, or 80μM
	Incubation Time:		24 hours
	Result:		Suppressed cyclin B1, cdc2 and cdc25c protein expression.
体内研究	Berberine (10, 30, or 50mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice
	Animal Model:		5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts
	Dosage:		10, 30, or 50mg/kg/day
	Administration:		Gastrointestinal gavage; for 10 consecutive days
	Result:		Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50mg/kg/day.

保存条件：

-20℃ 干燥

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果