中文名称: | 苄丝肼 盐酸盐 促销 | ||||
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英文名称: | Benserazide hydrochloride | ||||
别名: | 盐酸苄丝肼;苄丝肼 (S)-2-Amino-3-hydroxy-N'-(2,3,4-trihydroxybenzyl)propanehydrazide hydrochloride;2'-(2,3,4-Trihydroxybenzyl)-DL-serinohydrazide monohydrochloride | ||||
CAS No: | 14919-77-8 | 分子式: | C10H15N3O5.HCl | 分子量: | 293.7 |
CAS No: | 14919-77-8 | ||||
分子式: | C10H15N3O5.HCl | ||||
分子量: | 293.7 | ||||
MDL: | MFCD00078571 | EINEC: | 238-991-9 | ||
EINEC: | 238-991-9 |
基本信息
产品编号: |
B10016 |
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产品名称: |
Benserazide hydrochloride |
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CAS: |
14919-77-8 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
293.70 |
-20℃ |
一个月 |
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化学名: |
2'-(2,3,4-Trihydroxybenzyl)-DL-serinohydrazide monohydrochloride |
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Solubility (25°C): |
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体外:
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DMSO |
58mg/mL (197.48mM) |
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Ethanol |
1mg/mL warmed with 50ºC water bath (3.4mM) |
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Water |
58mg/mL (197.48mM) |
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体内(现配现用): |
1.请依序添加每种溶剂:PBS Solubility: 100mg/mL (340.48mM); Clear solution; Need ultrasonic 2.请依序添加每种溶剂:10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (7.08mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (7.08mM,饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加⼊ 50μL Tween-80,混合均匀;然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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3.请依序添加每种溶剂:10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08mg/mL (7.08mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (7.08mM,饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中,混合均匀。 |
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4.请依序添加每种溶剂:10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (7.08mM); Clear solution |
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此⽅案可获得 ≥ 2.08mg/mL (7.08mM,饱和度未知) 的澄清溶液,此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例,取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中,混合均匀。 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.4048mL |
17.0242mL |
34.0483mL |
5mM |
0.6810mL |
3.4048mL |
6.8097mL |
10mM |
0.3405mL |
1.7024mL |
3.4048mL |
50mM |
0.0681mL |
0.3405mL |
0.6810mL |
生物活性
产品描述 |
多巴脱羧酶抑制剂, |
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靶点 |
aromatic-L-amino-acid decarboxylase |
DOPA decarboxylase |
体外研究 |
Benserazide hydrochloride (BH) and Levodopa (LD) individually and in combination (Benserazide hydrochloride + LD) (25μM; 0 hour, 12 hours, 24 hours and 168 hours; SH-SY5Y) treatment inhibit protein aggregation and have the ability to minimise the amyloid-induced cytotoxicity in human neuroblastoma cell line. Benserazide hydrochloride and LD both can act as efficient inhibitors of the formation of cytotoxic HSA aggregates, and the inhibitory effects are more pronounced when both of these drugs are added simultaneously Cell Viability Assay |
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Cell Line: |
SH-SY5Y cells |
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Concentration: |
25μM |
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Incubation Time: |
0 hour, 12 hours, 24 hours and 168 hours |
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Result: |
Enhanced cell viability, and inhibited the formation of cytotoxic human serum albumin (HSA) aggregates. |
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体内研究 |
Benserazide (5-50mg/kg; intraperitoneal injection; male Wistar rats) treatment of 6-OHDA-lesioned rats increases exogenous L-DOPA-derived extracellular DA levels, the time to reach the peak DA levels are significantly prolong by Benserazide dosedependently. The AADC activity in the denervates striatal tissues shows a significant decrease by 10mg/kg and 50mg/kg Benserazide. Benserazide reduces the central AADC activity in the striatum of rats with nigrostriatal denervation, which leads to changes in the metabolism of exogenous L-DOPA |
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Animal Model: |
Male Wistar rats with 6-hydroxydopamine (6-OHDA) (8 Ag/4 Al) |
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Dosage: |
5mg/kg, 10mg/kg or 50mg/kg (Pharmacokinetic study) |
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Administration: |
Intraperitoneal injection |
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Result: |
Increased in exogenous L-DOPA-derived extracellular DA levels, the time to reach the peak DA levels were significantly prolonged in a dose-dependent fashion. The AADC activity in the denervated striatal tissues showed a significant decreased by 10mg/kg and 50mg/kg. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )