Acadesine - CAS:2627-69-2 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Acadesine
英文名称:	Acadesine
别名:	5-氨基-1-beta-D-呋喃核糖基-1H-咪唑-4-甲酰胺 Acadesine
CAS No:	2627-69-2	分子式:	C₉H₁₄N₄O₅	分子量:	258.23
CAS No:	2627-69-2
分子式:	C₉H₁₄N₄O₅
分子量:	258.23
MDL:	MFCD00869751	EINEC:	220-097-5
EINEC:	220-097-5

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A70007 Acadesine ≥98% (psaitong)

相关产品

熔点：

214-215°C

外观与性状：

白色或类白色粉末

包装规格：

25mg 100mg 1g in glass bottle

溶解性：

溶于水（ >7 mg/mL ）

产品描述：

基本信息

产品编号：A70007
产品名称：Acadesine
CAS:	2627-69-2	储存条件	粉末	-20℃	四年

分子式：	C9H14N4O5		溶于液体	-80℃	六个月
分子量：	258.23			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	2mg/mL
		Ethanol	Insoluble
		Water	25mg/mL
	体内（现配现用）	2% DMSO+40% PEG 300+2% Tween 80+ddH2O	6mg/mL
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.8725mL	19.3626mL	38.7252mL
5mM	0.7745mL	3.8725mL	7.7450mL
10mM	0.3873mL	1.9363mL	3.8725mL
50mM	0.0775mL	0.3873mL	0.7745mL

生物活性

产品描述	AICAR是AMP活化蛋白激酶(AMPK)的细胞渗透性激活剂，AMPK是一种代谢的主要调节因子，在能量可用性降低时(高细胞AMP:ATP比率)被激活，并抑制合成代谢过程。在体内，AMPK的药物激活与AICAR模拟运动，并触发骨骼肌胰岛素依赖型葡萄糖摄取。
靶点/IC50	AMPK (Cell-free assay)	AMPKK (Cell-free assay)
体外研究	HepG2 cells are treated with various concentrations of AICAR (0.1-1.0 mM) for 12, 24, and 48h,respectively. The expression level of IR-β significantly decreases with 0.25, 0.5, and 1.0mM of AICAR at 48h to 50%, 53%, and 46% of the control,respectively.
体内研究	Fourteen-week-old male, lean (L; 31.3g body wt) wild-type andob/ob (O; 59.6g body wt) mice are injected with the AMPactivated kinase (AMPK) activator AICAR (A) at 0.5mg/g per day or saline control (C) for 14 days. At 24h after the last injection (including a 12-h fast), all mice are killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) is excised for analysis. Muscle mass is lower in OC (159±12mg) than LC, LA, and OA (176±10, 178±9, and 166±16mg,respectively) mice, independent of a body weight change. The kidney weight is significantly higher in the untreated group when compared with both the exercise and AICAR (0.5mg/g body wt) groups. The heart weight is higher in the exercise group than in the other groups, whereas the liver weight is significantly higher in the AICAR-treated group when compared with the exercise and untreated groups.

推荐实验方法(仅供参考)

细胞实验:

HepG2 cells (5×105 cells) are plated in 6-well culture plate dishes and then are incubated in the serum-free media for 12h before transfection. One microgram of plasmid is transfected with FuGENE6 Transfection Reagent. After 5h of transfection,the culture media are removed and then media supplemented with or without AICAR (0.1-1.0mM) are added to each well.The stimulation media are changed every 24h.

动物实验:

Rats

Male 5-week-old ZDF rats are either subcutaneously injected with a single dose of AICAR (0.5mg/g body wt) or underwent a single bout of treadmill running (60 min, speed of 25m/min at a 5% incline). Untreated ZDF rats serve as controls (n=5 in each group). One hour after the subcutaneous AICAR injection or immediately after treadmill running, rats are killed by cervical dislocation. To avoid any effect of muscle spasm and hypoxia, red and white gastrocnemius muscles are removed within seconds and immediately freeze clamped for later determination of AMPK activity.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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