基本信息
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产品编号： |
A12799 |
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产品名称： |
Aconine |
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CAS: |
509-20-6 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
499.59 |
-20℃ |
1个月 |
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化学名： |
Jesaconine |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.0016mL |
10.0082mL |
20.0164mL |
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5mM |
0.4003mL |
2.0016mL |
4.0033mL |
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10mM |
0.2002mL |
1.0008mL |
2.0016mL |
生物活性
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产品描述 |
抑制核因子κB受体活化剂配体诱导的NF-κB激活。 |
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靶点 |
NF-κB |
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体外研究 |
Treatment with Aconine significantly inhibits the RANKL-induced transcriptional activity of NF-κB in a dose-dependent manner.Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing the activation of NF-κB and NFATc1 and the expression of the cell-cell fusion molecule DC-STAMP.Aconine (0.125,0.25μM) does not affect the viability of RAW264.7 cells,but dose dependently inhibits RANKL-induced osteoclast formation and bone resorptive activity.Aconine dose-dependently inhibits the RANKL-induced activation of NF-κB and NFATc1 in RAW264.7 cells,and subsequently reduces the expression of osteoclast-specific genes (c-Src,β3-Integrin,cathepsin K and MMP-9) and the expression of dendritic cell-specific transmembrane protein (DC-STAMP),which plays an important role in cell-cell fusion. |
推荐实验方法(仅供参考)
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细胞实验: |
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To evaluate the effect of Aconine on the viability of RAW264.7 cells,cytotoxicity assays are performed using the Cell Counting Kit-8.Briefly,the cells are seeded in 96-well plates at a density of 2×104,3×103,1.2×103,1×103 or 1×103 cells/well in the presence or absence of Aconine (0.125-0.5mM) for 8h,24h,48h,5 d or 7 d,respectively. After incubating the cells with CCK-8 solution for 2h,optical density is measured at 450nm using a GENios microplate reader.Cell viability is expressed as a percentage of the control. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
