基本信息
产品编号: |
A12799 |
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产品名称: |
Aconine |
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CAS: |
509-20-6 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
6个月 |
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分子量: |
499.59 |
-20℃ |
1个月 |
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化学名: |
Jesaconine |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.0016mL |
10.0082mL |
20.0164mL |
5mM |
0.4003mL |
2.0016mL |
4.0033mL |
10mM |
0.2002mL |
1.0008mL |
2.0016mL |
生物活性
产品描述 |
抑制核因子κB受体活化剂配体诱导的NF-κB激活。 |
靶点 |
NF-κB |
体外研究 |
Treatment with Aconine significantly inhibits the RANKL-induced transcriptional activity of NF-κB in a dose-dependent manner.Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing the activation of NF-κB and NFATc1 and the expression of the cell-cell fusion molecule DC-STAMP.Aconine (0.125,0.25μM) does not affect the viability of RAW264.7 cells,but dose dependently inhibits RANKL-induced osteoclast formation and bone resorptive activity.Aconine dose-dependently inhibits the RANKL-induced activation of NF-κB and NFATc1 in RAW264.7 cells,and subsequently reduces the expression of osteoclast-specific genes (c-Src,β3-Integrin,cathepsin K and MMP-9) and the expression of dendritic cell-specific transmembrane protein (DC-STAMP),which plays an important role in cell-cell fusion. |
推荐实验方法(仅供参考)
细胞实验: |
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To evaluate the effect of Aconine on the viability of RAW264.7 cells,cytotoxicity assays are performed using the Cell Counting Kit-8.Briefly,the cells are seeded in 96-well plates at a density of 2×104,3×103,1.2×103,1×103 or 1×103 cells/well in the presence or absence of Aconine (0.125-0.5mM) for 8h,24h,48h,5 d or 7 d,respectively. After incubating the cells with CCK-8 solution for 2h,optical density is measured at 450nm using a GENios microplate reader.Cell viability is expressed as a percentage of the control. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )