Ampkinone - CAS:1233082-79-5 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Ampkinone
英文名称:	Ampkinone
别名:	2-羟基雌二醇 Ampkinone;2-(4-benzoylphenyl)-6-hydroxy-7-methoxy-4,4-dimethylchromeno[3,4-e]isoindole-1,3-dione;2-[4-(benzoyl)phenoxy]-5-nitrobenzonitrile;AC1LAJGQ;AC1Q1XUL;AG-H-16433;Benzonitrile, 2-(4-benzoylphenoxy)-5-nitro-;CHEMBL334475;CTK5E6239
CAS No:	1233082-79-5	分子式:	C₃₁H₂₃NO₆	分子量:	505.52
CAS No:	1233082-79-5
分子式:	C₃₁H₂₃NO₆
分子量:	505.52

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

A11396 Ampkinone ≥98% (psaitong)

相关产品

包装规格：

2mg in glass bottle

溶解性：

溶于DMSO（50mg/mL 超声）

产品描述：

基本信息

产品编号：A11396
产品名称：Ampkinone
CAS:	1233082-79-5	储存条件	粉末	-20℃	四年

分子式：	C31H23NO6		溶于液体	-80℃	六个月
分子量：	505.52			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO
		Ethanol
		Water
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	1.9782mL	9.8908mL	19.7816mL
5mM	0.3956mL	1.9782mL	3.9563mL
10mM	0.1978mL	0.9891mL	1.9782mL

生物活性

产品描述	一种间接的 AMP 激活蛋白激酶 (AMPK) 激活剂。
靶点/IC50	AMPK
体外研究	Ampkinone stimulates the phosphorylation of AMPK via the indirect activation of AMPK in various cell lines.Ampkinonemediated activation of AMPK requires the activity of LKB1 and results in increased glucose uptake inmuscle cells.
体内研究	Ampkinone-treated DIO mice significantly reduce total body weight and overall fat mass.Histological examination and measurement of lipid parameters show that Ampkinone effectively improves metabolic abnormalities in the DIO mice model.

推荐实验方法(仅供参考)

激酶实验：

Total AMPK activity is measured using a synthetic SAMS peptide substrate and [γ-32P]ATP.Briefly,500μg of protein extract is incubated with anti-AMPK-α1 and α2 antibodies for 2h at 4℃.Protein A/G sepharose and agarose are added,and the mixtures are incubated for 3h at 4℃.After the samples are washed three times with RIPA buffer,the activity is assessed in AMPK reaction buffer containing 20mM HEPES-NaOH (pH 7.0),0.4mM Dithiothreitol, and 0.01% Brij-35,with or without 300μM AMP.The immune complexes are added to 23μL of reaction buffer per assay,and then 7μL of SAMS substrate peptide (HMRSAMSGLHLVKRR,100μM final concentration) and 10μL of ATP mixture (analiquot of 1μCi/μL: 90μL of 75mM magnesium chloride,500μM unlabeled ATP in 20mM MOPS,pH7.2,25mMβ-glycerophosphate,5mM EGTA,1mM sodium orthovanadate,and 1 mM Dithiothreitol) are added,and the mixture is incubated at 30℃ for 15 min.After 35μL is spotted onto the center of P81 paper,the paper is washed three times with 0.75% phosphoric acid and once with acetone for 5 min.The samples are read using a scintillation counter.

动物实验:

Mice

C57BL/6J mice are used and housed individually in a room maintained at 25℃ on a 12/12h light/dark schedule.For dietinduced obesity (DIO) mice,4-week-old male C57BL/6J mice are fed a high-fat diet (HFD,60% calories from fat) ad libitum for 8 weeks.Ampkinone in polyethylene glycol (PEG400) or vehicle is administered subcutaneously at 10mg/kg body weight per day for 1 month.Body weight and food intake are measured every 3 days.All animals are given an insulin tolerance test (ITT) and sacrificed at 30 days.Liver weight and fat masses are measured,sectioned,and stained with Oil-red O and H&E.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果