| 中文名称: | AGN 194310 | ||||
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| 英文名称: | AGN 194310 | ||||
| 别名: | VTP-194310 | ||||
| CAS No: | 229961-45-9 | 分子式: | C28H24O2S | 分子量: | 424.55 |
| CAS No: | 229961-45-9 | ||||
| 分子式: | C28H24O2S | ||||
| 分子量: | 424.55 | ||||
基本信息
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产品编号： |
A11324 |
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产品名称： |
AGN 194310 |
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CAS: |
229961-45-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
424.55 |
-20℃ |
一个月 |
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化学名： |
4-((4-(4-ethylphenyl)-2,2-dimethyl-2H-thiochromen-6-yl)ethynyl)benzoic acid |
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Solubility (25°C)： |
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体外:
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DMSO |
50mg/mL(117.77mM;Need ultrasonic) |
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Ethanol |
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Water |
<0.1mg/mL(insoluble) |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(5.89mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(5.89mM，饱和度未知)的澄清溶液。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到400μL PEG300中，混合均匀；向上述体系中加⼊50μL Tween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 |
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2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(5.89mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(5.89mM，饱和度未知)的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到900μL⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.3554mL |
11.7772mL |
23.5544mL |
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5mM |
0.4711mL |
2.3554mL |
4.7109mL |
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10mM |
0.2355mL |
1.1777mL |
2.3554mL |
生物活性
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产品描述 |
一种高亲和力的、有效的、具有选择性的视黄酸受体(RARs)的泛拮抗剂，对RARα，RARβ，RARγ的Kd值分别为3nM，2nM，5nM。 |
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靶点 |
RARα 3nM(Kd) |
RARβ 2nM(Kd) |
RARγ 5nM(Kd) |
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体外研究 |
AGN194310 potently inhibits colony formation by all three lines, with IC50 values of 16nM for LNCaP cells; 18nM for PC3 cells; and 34nM for DU-145 cells. AGN 194310 (50nM, 100nM; LNCaP, PC-3 and DU-145 cells) inhibits colony formation at concentrations of 50nM and 100nM alone and in combination with TTNPB. AGN 194310 (1μM; 72 hours; LNCaP cells) treatment results in 80% apoptosis Cell Viability Assay |
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Cell Line: |
LNCaP, PC-3 and DU-145 cells. |
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Concentration: |
50nM, 100nM |
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Incubation Time: |
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Result: |
When used together with 100nM TTNPB, there was almost complete reversal of the growth inhibitory effect of 50nM and partial reversal of the effect of 100nM. |
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Apoptosis Analysis |
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Cell Line: |
LNCaP cells |
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Concentration: |
1μM |
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Incubation Time: |
72 hours |
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Result: |
Induced apoptosis in LNCaP cells. |
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体内研究 |
AGN 194310 (0.5mg/kg/day; oral gavage; every day; for 10 days; female C57Bl/6J mice) treatment increases the number of granulocytes across haemopoietic compartments. A significant increase in the frequency of granulocyte-progenitor cells is observed in the bone marrow of mice after treatment with AGN194310 |
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Animal Model: |
Female C57Bl/6J mice (Five-week-old (34-37 days)) |
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Dosage: |
0.5mg/kg/day |
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Administration: |
Oral gavage; every day; for 10 days |
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Result: |
The number of granulocytes was significantly increased across haemopoietic compartments. Progenitor cells containing granulocytes also increased significantly. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
