A 83-01 - CAS:909910-43-6 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	A 83-01 促销
英文名称:	A 83-01
别名:	3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide
CAS No:	909910-43-6	分子式:	C₂₅H₁₉N₅S	分子量:	421.52
CAS No:	909910-43-6
分子式:	C₂₅H₁₉N₅S
分子量:	421.52

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A10095 A 83-01 ≥98% (HPLC) (psaitong)

相关产品

包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO(5mg/mL)

产品描述：

基本信息

产品编号：A10095
产品名称：A 83-01
CAS:	909910-43-6	储存条件	粉末	-20℃	四年
分子式：	C25H19N5S
分子量	421.52
化学名：
Solubility (25°C)	体外	DMSO	21mg/mL (49.81mM)
		Ethanol	Insoluble
		Water	Insoluble
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.3724mL	11.8618mL	23.7237mL
5mM	0.4745mL	2.3724mL	4.7447mL
10mM	0.2372mL	1.1862mL	2.3724mL

生物活性

产品描述	一种ALK抑制剂，能抑制TGF-βⅠ型受体ALK-5和活化素IB型受体ALK-4及结节型受体ALK-7诱导的转录活性，其激酶结构域与ALK-5结构域高度相关， IC50分别为 12nM、45nM和7.5nM。
靶点/IC₅₀	ALK5 12nM (IC50)	ALK4 45nM (IC50)	ALK7 7.5nM(IC50)
体外研究	A 83-01 is a potent inhibitor of TGF-β type I receptor ALK5 kinase, ALK4 and ALK7, reduces the level of ALK-5-induced transcription with an IC50 of 12nM in Mv1Lu cells, also blocks the ALK4-TD and ALK7-TD induced transcription with IC50s of 45nM and 7.5nM in R4-2 cells, and weakly suppresses that induced by constitutively active ALK-6, ALK-2, ALK-3, and ALK-1. A 83-01 (0.03-10µM) potently prevents the growth-inhibitory effects of TGF-β, and completely inhibits the effect at 3 µM. A 83- 01 (1-10µM) inhibits TGF-β-induced Smad activation in HaCaT cells[1]. A 83-01 (1µM) decreases cell motility, adhesion and invasion increased by TGF-β1 in HM-1 cells, but does not change cell proliferation.
体内研究	A 83-01 (50, 150 and 500μg/mouse, i.p.) significantly improves survival of the mice without body weight or neurobehavioral appearances. A 83-01 (0.5mg/kg, i.p.) shows a significantly strong antitumor effect in mice bearing M109 cells.

推荐实验方法(仅供参考)

细胞实验:
HM-1 cells are seeded into a 96-well plate and are incubated for 18 hr. A 83-01 (1μM) or vehicle are then added for 12 hr followed by the addition of TGF-β1 (1ng/mL) or vehicle for 60 hr. The number of viable cells in each well is examined using the WST-1 assay.

动物实验:

Mice
Female B6C3F1 mice used for the in vivo studies are maintained under specific pathogen-free conditions. To evaluate the effect of A 83-01 on the survival of mice bearing peritoneal dissemination, HM-1 cells(1×106 ) are injected into the abdominal cavity via the left flank of the mouse. Starting the next day, A 83-01 (150μg/body) or vehicles (PBS with 0.5% DMSO) are injected into the abdominal cavity three times per week. Mice are euthanized before reaching the moribund state.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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