| 中文名称: | β-淀粉状蛋白25-35 | ||||
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| 英文名称: | Amyloid β-Protein Fragment 25-35 | ||||
| 别名: | β-淀粉状蛋白25-35 Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met | ||||
| CAS No: | 131602-53-4 | 分子式: | C45H81N13O14S | 分子量: | 1060.27 |
| CAS No: | 131602-53-4 | ||||
| 分子式: | C45H81N13O14S | ||||
| 分子量: | 1060.27 | ||||
| MDL: | MFCD00133076 | ||||
基本信息
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产品编号： |
A10068 |
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产品名称： |
Amyloid β-Protein Fragment 25-35 |
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CAS: |
131602-53-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
1060.27 |
-20℃ |
一个月 |
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化学名： |
Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met |
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Solubility (25°C)： |
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体外:
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DMSO |
100mg/mL (94.32mM; Need ultrasonic) |
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Ethanol |
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Water |
3.33mg/mL (3.14mM; Need ultrasonic) |
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1.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (2.36mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (2.36mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
0.9432mL |
4.7158mL |
9.4316mL |
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5mM |
0.1886mL |
0.9432mL |
1.8863mL |
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10mM |
0.0943mL |
0.4716mL |
0.9432mL |
生物活性
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产品描述 |
β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) 是阿尔茨海默⽒淀粉样蛋⽩β肽的Aβ(25-35) ⽚段，在培养细胞中显⽰出 神经毒性活性。 |
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体外研究 |
The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine35, plays a key role in the induction of programmed cellular death pathways and toxic events. β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments. Methods vary depends on the downstream applications. |
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体内研究 |
Rats are injected with Aβ25–35 peptide intracerebroventricularly and compound Danshen (CDS) are subsequently administered once daily for 23 days. Rats’ behavior is monitored using Morris water maze and passive avoidance. Real time PCR and Western blotting are used in determining amyloid precursor protein (APP), β-site APP cleaved enzyme-1(BACE1), Presenilin-1 (PS1), Insulin-degrading enzyme (IDE) and neprilysin (NEP) in hippocampus. The Alzheimer' s disease (AD) model group present with spatial learning and memory impairments. CDS and donepezil administration significantly ameliorate the Aβ25–35 peptide-induced memory impairment in both Morris water maze (P < 0.05) and passive avoidance task (P < 0.01) compared to the AD model group. |
推荐实验方法(仅供参考)
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Cell Assay |
Cell viability is determined by a modified MTS assay, which is based on the conversion of Tetrazolium salt by mitochondrial dehydrogenase to a formazan product spectrophotometrically measurable at 490nm. PC12 cells are plated in 96-well plates at a density of 10 000 cells/well and maintained for 16 h in complete medium. Cells are then incubated in the absence (control) and presence of 40μM Aβ(31-35) and Aβ(25-35) with reduced, oxidized and norleucine-substituted methionine-35 staurosporine 10μM is used as positive control of 100% of cellular death. After 48 h of peptide-incubation, 20μL of MTS reagent (2.0mg/mL) is added to each well. The cells are then incubated for 30-45 min at 37 °C in a 5% CO2 incubator. The reaction is stopped by adding 25μL of 10% SDS. The plates are read with a microplate reader at 490nm. Each data point is obtained using a triplet-well assay |
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Animal Administration |
Rats Fifty-four Male Sprague-Dawley rats (2 months old, 300-350 g) are used. Amyloid beta-peptide(25-35) is dissolved in sterile distilled water at a concentration of 1mg/mL as a stocking solution. Animals are infused with 5μL/side of sterile distilled water (control), aggregated Aβ25-35 (2μg/μL), into bilateral cerebral lateral ventricles at a rate of 1μL/min; the needle is left in place for 5 min. Then the needles are removed and rats are kept on a warm pad until they are awakened. To determine the neuroprotective effect on AD rats, the Aβ25-35 treated rats are treated with CDS of different doses and Donepezil once daily for 23 days (including duration of behavior test). Experiment is performed to test the effect of CDS on Aβ25-35-induced memory impairment using Morris water-maze and step-through passive avoidance tasks. Specifically, all of the rats are randomly divided into 6 groups for the experiment: (a) Vehicle 1 (for Aβ25-35)+vehicle 2 (for CDS and Donepezil), (b) Aβ25-35 +vehicle 2, (c) Aβ25-35+CDS (130mg/kg), (d) Aβ25-35+CDS (260mg/kg), (e) Aβ25-35+CDS (520mg/kg), (f) Aβ25-35+Donepezil (0.5mg/kg). One day after cerebroventricular microinfusions of Aβ25-35 (10μg/side) or its vehicle, rats are treated (i.g.) with CDS or Donepezil or vehicle 2, once daily for 14 days prior to the beginning of Morris water maze, followed by passive avoidance task. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
