Z-VAD-FMK - CAS:187389-52-2 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Z-VAD-FMK
英文名称:	Z-VAD-FMK
别名:	2-氯苯丙胺; N-Benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone；Z-Val-Ala-Asp(OMe)-FMK
CAS No:	187389-52-2	分子式:	C₂₂H₃₀FN₃O₇	分子量:	467.49
CAS No:	187389-52-2
分子式:	C₂₂H₃₀FN₃O₇
分子量:	467.49

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Z10006 Z-VAD-FMK 95% (psaitong)

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包装规格：

2mg 10mg in glass bottle

溶解性：

溶于DMSO(20mg/mL )

产品描述：

基本信息

产品编号：Z10006
产品名称：Z-VAD-FMK
CAS:	187389-52-2	储存条件	粉末	-20℃	四年

分子式：	C22H30FN3O7		溶于液体	-80℃	六个月
分子量	467.49			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	93mg/mL (198.93mM)
		Ethanol	2mg/mL warmed with 50ºC water bath (4.27mM)
		Water	Insoluble
	体内（现配现用）	2% DMSO+35 %PEG 300+2%Tween 80+ddH2O	6mg/mL
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.1391mL	10.6954mL	21.3908mL
5mM	0.4278mL	2.1391mL	4.2782mL
10mM	0.2139mL	1.0695mL	2.1391mL
50mM	0.0428mL	0.2139mL	0.4278mL

生物活性

产品描述	Z-VAD(OMe)-FMK (Z-Val-Ala-Asp(OMe)-FMK) 是一种不可逆的 pan-caspase 抑制剂。Z-VAD(OMe)-FMK 是泛素 C 末端水解酶 L1 (UCHL1) 抑制剂。Z-VAD(OMe)-FMK 通过靶向 UCHL1 活性位点对 UCHL1 进行不可逆地修饰。
靶点/IC50	Pan-caspase (THP.1, Jurkat T-cells)
体外研究	Z-VAD(OMe)-FMK (Z-Val-Ala-Asp(OMe)-FMK) is a broad-spectrum caspase inhibitor, has been shown to inhibit the intracellular activation of caspase-like proteases. The injection of Z-VAD(OMe)-FMK suppresses the caspase-3 activity in lung tissues, and significantly decreases the number of terminal dUTP nick-end labeling-positive cells.Z-VAD(OMe)-FMK effectively inhibits UCHL1's reaction with hemagglutinin-tagged ubiquitin vinylmethyl ester (HA-UbVME) at the concentration of 100μM.Z-VAD(OMe)-FMK is administered intraperitoneally at 1 hour before and 6 hours after SAH.Expression of caspase-3 and positive TUNEL is examined as markers for apoptosis. Z-VAD(OMe)-FMK suppresses TUNEL and caspase-3 staining in endothelial cells,decreases caspase-3 activation, reduces BBB permeability, relieves vasospasm,abolishes brain edema, and improves neurological outcome.Z-VAD(OMe)-FMK is a cell-permeable caspase inhibitor,efficiently blocks cell death induced by SMN deficiency.
体内研究	The survival rate of mice is prolonged significantly by the injection of Z-VAD(OMe)-FMK (Z-Val-Ala-Asp(OMe)-FMK). All mice succumbed to LPS within 30 hours. By contrast, the mice treated with Z-VAD(OMe)-FMK survive significantly longer and 27% of the mice survived more than 7 days.

推荐实验方法(仅供参考)

细胞实验:

PCR products containing coding sequences for the dSMN (forward primer: 5′-TAA TAC GAC TCA CTA TAG GG AAG ACG TAC GAC GAG TCG-3′; and reverse primer: 5′-TAA TAC GAC TCA CTA TAG GG GTG GTG CTG GCT TCT TTC-3′; product length,601bps; bold and italics letters represent T7 promoter sequences) and control Drosophila Presenilin (dPsn) gene (forward primer: 5′-TAA TAC GAC TCA CTA TAG GG TG GCT GCT GTC AAT CTC-3′; and reverse primer: 5′-TAA TAC GAC TCA CTA TAG GG CGA TAG CAA CGC TTC TTG-3′; product length: 543bps) are obtained and gel-purified. Double-stranded RNAs (dsRNA) are generated by transcription with Ribomax T7 Transcription kit and digested with Rnase-free DNase. The dsRNA products are ethanol precipitated and annealed by incubation at 65℃ for 30 min and then slowly allowed to cool at room temperature.The annealed dsRNA products are analyzed on a 1% agaorse gel to ensure the majority of dsRNA existed as a single band.The dsRNA (2μg) and/or plasmid DNAs (2μg) are introduced into cells by using Cellfectin. Caspase inhibition is achieved by using 50μM of Z-VAD(OMe)-FMK in the culture medium.

动物实验:

Mice

Mice used in this study are 5- to 6-week-old (20 to 22g) ICR males. Mice are injected with 30mg/kg LPS from E. coli serotype O111:B4 through the tail vein. A single intravenous injection of Z-VAD(OMe)-FMK (0.25mg) is made 15 minutes before LPS injection, followed by three intravenous injections of Z-VAD(OMe)-FMK (0.1mg each) per hour. Control mice are injected with the same volume of 1% DMSO in sterile saline.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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