S-2474 - CAS:158089-95-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	S-2474
英文名称:	S-2474
别名:	S-2474 (E)-5-(3,5-di-tert-butyl-4-hydroxybenzylidene)-2-ethylisothiazolidine 1,1-dioxide
CAS No:	158089-95-3	分子式:	C₂₀H₃₁NO₃S	分子量:	365.53
CAS No:	158089-95-3
分子式:	C₂₀H₃₁NO₃S
分子量:	365.53

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S11007 S-2474 (psaitong)

相关产品

包装规格：

100mg in glass bottle

产品描述：

基本信息

产品编号：	S11007
产品名称：	S-2474
CAS:	158089-95-3	储存条件	粉末	-20℃	四年
分子式：	C20H31NO3S		溶于液体	-80℃	二年
分子量：	365.53
化学名：	(E)-5-(3,5-di-tert-butyl-4-hydroxybenzylidene)-2-ethylisothiazolidine 1,1-dioxide
Solubility (25°C)：
体外:	DMSO
	Ethanol
	Water
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

生物活性

产品描述

COX-2和5-lipoxygenase(5-LO)的抑制剂，在人完整的细胞中，对COX-2和COX-1的IC50值分别为11nM和27μM，为一种非甾体抗炎药。

靶点

COX-2 11nM (IC50)

COX-1 27μM (IC50)

5-LO

体外研究

S-2474 is an inhibitor of COX-2 and 5-lipoxygenase,with IC50s of 11nM and 27μM for COX-2 and COX-1.S-2474 prevents neurons from Aβ-induced cell death significantly in a concentration-dependent manner (IC50 =26±12nM).S-2474 (10μM) completely inhibits Aβ(25-35)-induced neuronal cell death.S-2474 also shows neuroprotective effects in the Aβ(1-40)-induced neuronal cell death.S-2474 inhibits the PGD2 generation in a concentration dependent manner (IC50=69.8±21.9nM).S-2474 (10μM) lowers the elevated level of PGD2 significantly and reduces radicals from Aβ(25-35)-treated neurons.S-2474 significantly prevents neurons from undergoing sPLA2-IIA-induced cell death.S-2474 completely ameliorates sPLA2-IIA-induced apoptotic features such as the condensation of chromatin and the fragmentation of DNA.Moreover,S-2474 significantly inhibits the sPLA2-IIA-induced generation of PGD2.S-2474 inhibits sPLA2-IIA-induced neuronal cell death in a concentration-dependent manner (IC50=94nM).

推荐实验方法(仅供参考)

细胞实验:

Experiments are principally performed in the two conditions as follows.(i) Neurons (2.5×105 cells/cm2) are treated with 10μM Aβ(25-35) or Aβ(1-40) in the presence or absence of S-2474 at 37℃.Vehicle controls are treated with culture medium containing 1% deionized water and 0.1% DMSO.Aβ controls are treated with culture medium containing 10μM Aβ(25-35) and 0.1% DMSO.(ii) Neurons (2.5×105 cells/cm2) are treated with eicosanoids at 37℃.Vehicle controls are treated with culture medium containing 0.1% ethanol.Two different methods are employed for assessment of neurotoxicity of Aβ.First,the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye (MTT) reduction assay reflecting mitochondrial succinate dehydrogenase activity is employed.Second,residual cells are counted according to morphologic criteria;neurons with intact neurites and a smooth,round soma are considered viable,whereas those with degenerated neurites and an irregular soma are considered nonviable.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果