| 中文名称: | SN6 | ||||
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| 英文名称: | SN6 | ||||
| 别名: | SN6 SN6 | ||||
| CAS No: | 415697-08-4 | 分子式: | C20H22N2O5S | 分子量: | 402.46 |
| CAS No: | 415697-08-4 | ||||
| 分子式: | C20H22N2O5S | ||||
| 分子量: | 402.46 | ||||
基本信息
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产品编号： |
S10966 |
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产品名称： |
SN6 |
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CAS: |
415697-08-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
402.46 |
-20℃ |
1个月 |
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化学名： |
2-[[4-[(4-NITROPHENYL)METHOXY]PHENYL]METHYL]-4-THIAZOLIDINECARBOXYLIC ACID ETHYL ESTER |
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Solubility (25°C)： |
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体外:
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DMSO |
80mg/mL (198.77mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.4847mL |
12.4236mL |
24.8472mL |
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5mM |
0.4969mL |
2.4847mL |
4.9694mL |
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10mM |
0.2485mL |
1.2424mL |
2.4847mL |
生物活性
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产品描述 |
一种选择性的NCX抑制剂 |
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靶点 |
IC502.9μM (NCX1),16μM (NCX2),8.6μM (NCX3) |
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体外研究 |
SN 6 is a selective Na+/Ca2+ exchanger inhibitor,which inhibits the initial rate of 45Ca2+ uptake into NCX1,NCX2,and NCX3 transfectants with IC50 values of 2.9±0.12,16±1.1,and 8.6±0.27μM.SN 6 (up to 30μM) also less potently inhibits muscarinic acetylcholine receptor,with a higher IC50 of 18μM.SN 6 (0.3-30μM) completely inhibits the initial rate of Na+i-dependent 45Ca2+ uptake into Na+loaded sarcolemmal vesicles in a dose dependent manner (IC50,5.3±0.37μM).SN 6 (0.3-10μM) dose-dependently protects against the hypoxia/reoxygenation-induced LDH release in parental LLC-PK1 cells and NCX1 transfectants but not in K229Q transfectants.SN 6 (1-30μM) suppresses the bidirectional outward and inward INCX in a concentration-dependent manner,with IC50 values of 2.3μM and 1.9μM,respectively.SN 6 also inhibits bidirectional current (INCX) in a [Na+]i concentration-dependent manner,with IC50 values of 3.4μM,2.3μM,and 1.1μM at 10mM,20mM,and 30mM [Na+]i,respectively.SN 6 inhibits hypoxia/reoxygenation-induced LDH release with an IC50 value of 0.63±0.15μM in NCX1 transfectants. |
推荐实验方法(仅供参考)
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细胞实验: |
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Na+i-dependent 45Ca2+ uptake into cells expressing the wild-type or mutated exchangers are assayed.In brief,confluent transfectants in 24-well dishes are loaded with Na+ by incubation at 37℃ for 40 min in 0.5mL of balanced salt solution (BSS) (10mM HEPES/Tris,pH 7.4,146mM NaCl,4mM KCl,2mM MgCl2,0.1mM CaCl2,10mM glucose,and 0.1% bovine serum albumin) containing 1mM ouabain and 10μM monensin.45Ca2+ uptake is then initiated by switching the medium to Na+-free BSS (replacing NaCl with equimolar choline chloride) or to normal BSS,both of which contain 0.1mM 45CaCl2 (370 kBq/mL) and 1mM ouabain.After a 30-s incubation,45Ca2+ uptake is terminated by washing cells four times with an ice-cold solution containing 10mM HEPES/Tris,pH 7.4,120mM choline chloride,and 10mM LaCl3.Cells are then solubilized with 0.1 N NaOH,and aliquots are taken for determination of radioactivity and protein.When present, SN 6 and KB-R7943 are included in the medium 15 min before the start of 45Ca2+ uptake. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
