| 中文名称: | SQ22536 促销 | ||||
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| 英文名称: | SQ22536 | ||||
| 别名: | 3-乙炔基-苯甲腈;9-(四氢-2-呋喃)腺膘呤 9-(tetrahydrofuran-2-yl)-9h-purin-6-amine;9-(Tetrahydrofuryl)adenine | ||||
| CAS No: | 17318-31-9 | 分子式: | C9H11N5O | 分子量: | 205.22 |
| CAS No: | 17318-31-9 | ||||
| 分子式: | C9H11N5O | ||||
| 分子量: | 205.22 | ||||
| EINEC: | 53339 | ||||
| EINEC: | 53339 | ||||
基本信息
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产品编号： |
S10833 |
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产品名称： |
SQ22536 |
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CAS: |
17318-31-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
205.22 |
-20℃ |
1个月 |
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化学名： |
9-(Tetrahydro-2-furanyl)-9H-purin-6-amine,9-THF-Ade;SQ-22536 |
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Solubility (25°C)： |
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体外:
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DMSO |
41mg/mL (199.78mM) |
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Ethanol |
41mg/mL (199.78mM) |
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Water |
41mg/mL (199.78mM) |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
4.8728mL |
24.3641mL |
48.7282mL |
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5mM |
0.9746mL |
4.8728mL |
9.7456mL |
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10mM |
0.4873mL |
2.4364mL |
4.8728mL |
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50mM |
0.0975mL |
0.4873mL |
0.9746mL |
生物活性
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产品描述 |
一种有效的腺苷酸环化酶 (AC) 抑制剂。 |
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靶点 |
adenylate cyclase (AC) |
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体外研究 |
SQ22536 (SQ22,536) effectively inhibits the effect of forskolin with respective IC50 values of 5μM.Preincubation with graded concentrations of SQ22536 reveals that both SQ22536 effectively inhibits PACAP-induced reporter gene activation with approximate IC50 value of 5μM.SQ22536 more potently inhibits forskolin-induced Elk activation (IC50=10μM) than 8-BrcAMP-induced Elk activation (IC50=170μM).Most notably,there are substantial differences in the reported potencies of SQ22536 to inhibit the activities of recombinant AC5 and AC6,with respective IC50 values of 2μM and 360μM.At a greater concentration (500μM),SQ22536 significantly inhibits neurite elongation due to either forskolin or 8-Br-cAMP. |
推荐实验方法(仅供参考)
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细胞实验: |
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HEK293 CRE-luc2P GloResponse luciferase reporter cells are transduced with retroviral vectors expressing rat PAC1hop receptors. Individual cell lines are obtained by limiting dilution cloning,and a clonal PAC1-expressing line is propagated and used for CRE luciferase assays.In brief, HEK293 CRE-luc2P cells are plated in 96-well plates (10,000 cells in 80μL media per well) in assay media (DMEM supplemented with 1% fetal bovine serum).One day after plating,cells are treated with AC inhibitors (10μL in assay media/well) for 30 minutes,followed by agonists (10μL in assay media/well),and are incubated for 4 hours.Luciferase activity is determined after the addition of 100μL/well Bright-Glo Luciferase Assay Reagent.Luminescence (RLU) is measured in a Victor3 microtiter plate reader after 2 minutes of agitation at room temperature.Cyclic AMP is measured in NS-1 cells. In brief,NS-1 cells are seeded and grown overnight in 96-well plates.The next day,cells are pretreated for 20 minutes in media containing the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.5mM) with or without SQ22536.After pretreatment with inhibitors,cells are stimulated with agonists, added as 10× solutions, for an additional 20 minutes.Intracellular cAMP is then assayed using the cAMP Biotrak enzyme immunoassay kit for measurement of nonacetylated cAMP. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
