中文名称: SKI V
英文名称: SKI V
CAS No: 24418-86-8
分子式: C15H10O4
分子量: 254.24
S10715 SKI V ≥98% (psaitong)
包装规格:
1mg 5mg 10mg in glass bottle
溶解性:
溶于DMSO(250 mg/mL 超声)
产品描述:

基本信息

产品编号:

S10715

产品名称:

SKI V

CAS:

24418-86-8

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C15H10O4

溶于液体

-80℃

6个月

分子量:

254.24

-20℃

1个月

化学名: 

2-(3,4-Dihydroxy-benzylidene)-benzofuran-3-one,Sphingosine Kinase Inhibitor V

Solubility (25°C):

 

体外:

 

DMSO

51mg/mL (200.59mM)

Ethanol

Insoluble

Water

Insoluble

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

3.9333mL

19.6665mL

39.3329mL

5mM

0.7867mL

3.9333mL

7.8666mL

10mM

0.3933mL

1.9666mL

3.9333mL

50mM

0.0787mL

0.3933mL

0.7867mL

 

生物活性

产品描述

一种非竞争性的,有效的非脂质鞘氨醇激酶(SPHK;SK)抑制剂,对GST-hSK的IC50为2μM。

靶点

GST-hSK 

hPI3k 

2μM

6μM

 

体外研究

SKI V has weak activity toward ERK2 (IC50 of 80μM for hERK2) and does not inhibit PKC-α.SKI V (10μM;for 24 hours) inhibits cancer cell proliferation and induces apoptosis.SKI V (0.2,1,5μM;pretreated for 1 hour) decreases phospho-Akt and phospho-MEK levels.Near-confluent cultures of JC cells are serum-starved for 16 hours,followed by pretreatment SKI V for 1 hour.SKI V has IC50s for inhibition of sphingosine kinase (SK) and tumor cell proliferation of -2μM.SKI V (20μg/ml) inhibits not only purified but endogenous SK in in MDA-MB-231 cells.SKI V (0.2,1,5μM) inhibits intracellular S1P formation in JC cells in a dose-dependent fashion.

Cell Proliferation Assay

Cell Line:

T24 tumor cells

Concentration:

10μM

Incubation Time:

For 24 hours

Result:

Inhibited cancer cell proliferation.

Apoptosis Analysis

Cell Line:

T24 tumor cells

Concentration:

10μM

Incubation Time:

For 24 hours

Result:

Induced apoptosis.

Western Blot Analysis

Cell Line:

JC cells

Concentration:

0.2,1,5μM

Incubation Time:

Pretreated for 1 hour

Result:

Decreased phospho-Akt and phospho-MEK levels.

体内研究

SKI V (75mg/kg;i.p.;days 1,5,9,15) significantly lowers tumor growth (>50% decreased at day 18) than control animals.

Animal Model:

6-8 weeks old BALB/c female mice with JC mammary adenocarcinoma cells

Dosage:

75mg/kg

Administration:

IP;days 1,5,9,15

Result:

Tumor growth was significantly lower (>50% decreased at day 18) than tumor growth in control animals.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):