星孢菌素 - CAS:62996-74-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	星孢菌素热销
英文名称:	Staurosporine
别名:	星形孢菌素 AM2282 CGP41251 Antibiotic 230
CAS No:	62996-74-1	分子式:	C₂₈H₂₆N₄O₃	分子量:	466.53
CAS No:	62996-74-1
分子式:	C₂₈H₂₆N₄O₃
分子量:	466.53
MDL:	MFCD00077402

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S10103 星孢菌素 ≥98%(HPLC) (psaitong)

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包装规格：

1mg 5mg 25mg in glass bottle

溶解性：

溶于DMSO(25mg/ml)和DMF(25mg/ml)

产品描述：

基本信息

产品编号：S10103
产品名称：Staurosporine
CAS:	62996-74-1	储存条件	粉末	-20℃	四年

分子式：	C28H26N4O3		溶于液体	-80℃	六个月
分子量：	466.53			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	46mg/mL (98.6mM)
		Ethanol	Insoluble
		Water	Insoluble
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.1435mL	10.7174mL	21.4348mL
5mM	0.4287mL	2.1435mL	4.2870mL
10mM	0.2143mL	1.0717mL	2.1435mL
50mM	0.0429mL	0.2143mL	0.4287mL

生物活性

产品描述	一种具有抗真菌活性的微生物碱,抑制多种蛋白激酶，包括PKA(Ki=7.0nM IC50=15nM)、PKG(Ki=8.5nM)、MYLK(MLCK，Ki=1.3nM)、PKC(Ki=0.7nM)、CaMK(IC50=20nM)、CAMKII(Ki=20nM)、酪氨酸激酶(IC50=70nM)和磷酸化酶激酶(IC50=0.5nM)。
靶点/IC50	PKCα (Cell-free assay)	c-Fgr (Cell-free assay)	phosphorylase kinase (Cell-free assay)	PKCη (Cell-free assay)	PKCγ (Cell-free assay)
靶点/IC50	2nM	2nM	3nM	4nM	5nM
体外研究	Staurosporine, widely used as a protein kinase C (PKC) inhibitor with a broad spectrum of activity, is an alkaloid isolated from the culture broth of Streptomyces staurospores. MC3T3E-1 osteoblasts, expose to Staurosporine (100nM) for 12 h,release an amount of LDH (12.4±3.1%) that is similar to that release by the control cells(10.0±2.4%), indicating the relative absence of lytic death, which occurs in necrosis. In addition, treatment with Staurosporine (100nM) results in morphological changes, characteristic of apoptosis: a brightblue fluorescent condensed nuclei seen through a fluorescence microscope after Hoechst 33258-staining, and a reduction of cell volume.
体内研究	The inhibitory effect of Staurosporine is statistically significant at around Wk 10 of tumor promotion. Although statistically significant inhibition is not obtained with 10ng of Staurosporine in later weeks of the experiment, a decreasing tendency in the percentages of tumor bearing mice and in average numbers of tumors per mouse is apparent.Thus,Staurosporine slightly inhibits tumor promotion of Teleocidin, even at the dose at which Staurosporine itself induced tumors.Staurosponne (0.05 and 0.1mg/kg intraperitoneal) attenuates the impaired perlormance of water maze and passive avoidance tasks, even though the drug administration began 2 weeks after the lesion.Moreover,Staurosporine (0.1mg/kg) partially reversed the decrease of choline acetyltransferase activity in the fronto-parietal cortex induced by basal forebrainlesion.These results suggest that Staurosporine attenuates impairment of learning through reversal of damage to cholinergic neurons induced by basal forebrain-lesion.

推荐实验方法(仅供参考)

细胞实验:
细胞系	PC12
浓度	溶于DMSO,终浓度为1μM
处理时间	~32小时
方法	使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合，然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞，测定凋亡细胞百分数。

动物实验:

Mice

Female CD-I mice are used. Various amounts of Staurosporine in 10μL of acetone are applied to the ears of 8-wk-old CD-I mice. The extent of irritation is expressed as the minimum dose of the compound causing irritation. Induction of HOC in Mouse Skin Staurosporine in 0.1mL of acetone is applied to the skin of the backs of CD-I mice, and a crude enzyme extract is obtained from the skin 18h later. HDC activity is expressed as pmol of CO2 released per mg of protein per lh of incubation.Induction of ODC in Mouse Skin Staurosporine in 0.2mL of acetone is applied to the skin of the backs of CD-I mice. After 4h,a crude enzyme extract is prepared from the epidermis,and its ODC activity is measured. Enzyme activity is expressed as nmol of CO2 per mg of protein per 30 min of incubation.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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