中文名称: | 利妥昔单抗 | ||||
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英文名称: | Rituximab | ||||
别名: | 利妥昔单抗 Anti-Human CD20 type I, Chimeric Antibody | ||||
CAS No: | 174722-31-7 | 分子式: | C6416H9874N1688O1987S44 | ||
CAS No: | 174722-31-7 | ||||
分子式: | C6416H9874N1688O1987S44 |
基本信息
产品编号:R10461 |
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产品名称:Rituximab |
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CAS: |
174722-31-7 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
生物活性
产品描述 |
一种抗 CD20 嵌合单克隆抗体,用于某些自身免疫疾病和癌症的研究。 |
靶点/IC50 |
CD20 |
体外研究 |
Rituximab inhibits the proliferation of stimulated human B cells, which is associated with a relative increase of B cells with an activated naive phenotype. Aside from this population shift, there are no major changes in phenotype or cytokine profile of the various B-cell subsets. B cells stimulated in the presence of rituximab induces stronger T-cell proliferation, compared to B cells stimulated in the absence of rituximab. All lymphoma cells tested are equally sensitive to antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-mediated phagocytosis of tumor cells,and rituximab-induced apoptosis.Rituximab induces high CDC killing of follicular lymphoma cells. |
体内研究 |
A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor, cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent. |
推荐实验方法(仅供参考)
激酶实验: |
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生化激酶实验测定IC50和动力学研究 |
IC50测量使用poly-Glu–Tyr(4:1)或者poly-Lys–Tyr(4:1,针对Lck)为底物。二价阳离子为20mM MgCl2(对于Src、Fyn、Yes、Lyn、Csk、Frk或Abl)或10mM MnCl(对于FGFR1、IGF1R、Lck或Met)。ATP终浓度为:Src,10μM;Fyn,6μM;Yes,100μM;Lyn,2μM;Csk,10μM;Frk,10μM;Abl,4μM;FGFR1,10μM;IGF1R,2μM;Lck,2μM;Met,5μM;PDGFR,6μM。PDGFRb的磷酸化IC50值是通过测量免疫沉淀的PDGFRb确定的。Km值使用Eadie-Hofstee法计算。 |
细胞实验: |
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A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor,cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent. |
动物实验: |
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C57BL/6 mice (8-10 wk of age) are inoculated 8×103 EL4-CD20+cells in 200μL of saline by tail vein injection. In parallel groups of mice,150g of rituximab, murine anti-CD20 IgG2a Ab 1F5, or control anti-human IL-2R Ab daclizumab in 300μL of saline, or saline only is inoculated i.p. 24h later. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )