中文名称: 利妥昔单抗
英文名称: Rituximab
CAS No: 174722-31-7
分子式: C6416H9874N1688O1987S44
R10461 利妥昔单抗 ≥95%,12mg/mL (psaitong)
包装规格:
1mg 5mg 25mg 100mg in glass bottle
产品描述:

基本信息

产品编号:R10461

产品名称:Rituximab

CAS:

174722-31-7

 

储存条件

粉末

-20℃

四年

分子式:

C6416H9874N1688O1987S44

溶于液体

-80℃

六个月

分子量

 

 

 

化学名: 

 

 

Solubility (25°C)

 

体外

DMSO

 

Ethanol

 

Water

 

体内(现配现用)

 

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

生物活性

产品描述

一种抗 CD20 嵌合单克隆抗体,用于某些自身免疫疾病和癌症的研究。

靶点/IC50

CD20

 

体外研究

Rituximab inhibits the proliferation of stimulated human B cells, which is associated with a relative increase of B cells with an activated naive phenotype. Aside from this population shift, there are no major changes in phenotype or cytokine profile of the various B-cell subsets. B cells stimulated in the presence of rituximab induces stronger T-cell proliferation, compared to B cells stimulated in the absence of rituximab. All lymphoma cells tested are equally sensitive to antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-mediated phagocytosis of tumor cells,and rituximab-induced apoptosis.Rituximab induces high CDC killing of follicular lymphoma cells.

体内研究

A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor, cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent.

 

推荐实验方法(仅供参考)

激酶实验:

生化激酶实验测定IC50和动力学研究

IC50测量使用poly-Glu–Tyr(4:1)或者poly-Lys–Tyr(4:1,针对Lck)为底物。二价阳离子为20mM MgCl2(对于Src、Fyn、Yes、Lyn、Csk、Frk或Abl)或10mM MnCl(对于FGFR1、IGF1R、Lck或Met)。ATP终浓度为:Src,10μM;Fyn,6μM;Yes,100μM;Lyn,2μM;Csk,10μM;Frk,10μM;Abl,4μM;FGFR1,10μM;IGF1R,2μM;Lck,2μM;Met,5μM;PDGFR,6μM。PDGFRb的磷酸化IC50值是通过测量免疫沉淀的PDGFRb确定的。Km值使用Eadie-Hofstee法计算。

 

细胞实验:

 

A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor,cures 100% of the animals.Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent.

 

动物实验:

 

C57BL/6 mice (8-10 wk of age) are inoculated 8×103 EL4-CD20+cells in 200μL of saline by tail vein injection. In parallel groups of mice,150g of rituximab, murine anti-CD20 IgG2a Ab 1F5, or control anti-human IL-2R Ab daclizumab in 300μL of saline, or saline only is inoculated i.p. 24h later.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):