中文名称: RG 13022
英文名称: RG 13022
CAS No: 136831-48-6
分子式: C16H14N2O2
分子量: 266.3
R10128 RG 13022 ≥98% (psaitong)
包装规格:
5mg 25mg 100mg in glass bottle
产品描述:

基本信息

产品编号:R10128

产品名称:RG 13022

CAS:

136831-48-6

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C16H14N2O2

溶于液体

-80℃

六个月

分子量

266.30

-20℃

一个月

化学名: 

 

 

Solubility (25°C)

 

体外

DMSO

53mg/mL (199.03mM)

Ethanol

5mg/mL (18.77mM)

 

Water

Insoluble

体内(现配现用)

 

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

3.7553mL

18.7765mL

37.5530mL

5mM

0.7511mL

3.7553mL

7.5106mL

10mM

0.3755mL

1.8777mL

3.7553mL

50mM

0.0751mL

0.3755mL

0.7511mL

 

生物活性

产品描述

一种EGF抑制剂,在HT-22细胞中IC50值为1μM。

靶点/IC50

EGFR
(Cell-free assay)

4μM

 

体外研究

RG13022 suppresses EGF-stimulated cancer cell proliferation. In a cell-free reaction RG13022 inhibits the autophosphorylation reaction of the EGF receptor in immunoprecipitates with an IC50 of 4μM. RG13022 inhibits colony formation and DNA synthesis by HER 14 cells, which were stimulated by 50 ng/mL EGF, in a dose-dependent manner. The IC 50s are 1 and 3μM for HER 14 colony formation and DNA synthesis, respectively. RG-13022 inhibits not only EGF-induced growth but also growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. RG-13022 also totally blocks estrogen-stimulated phosphorylation of the EGF receptor, as well as estrogen-induced cell proliferation, suggesting that functioning TK pathways are required for estrogen action.

 

体内研究

RG13022 suppresses tumor growth in nude mice. RG13022 also increases the life span of these tumor-bearing nude mice.

 

推荐实验方法(仅供参考)

细胞实验:

 

RG13022 suppresses EGF-stimulated cancer cell proliferation. In a cell-free reaction RG13022 inhibits the autophosphorylation reaction of the EGF receptor in immunoprecipitates with an IC50 of 4μM. RG13022 inhibits colony formation and DNA synthesis by HER 14 cells, which were stimulated by 50ng/mL EGF, in a dose-dependent manner. The IC 50s are 1 and 3μM for HER 14 colony formation and DNA synthesis, respectively. RG-13022 inhibits not only EGF-induced growth but also growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. RG-13022 also totally blocks estrogen-stimulated phosphorylation of the EGF receptor, as well as estrogen-induced cell proliferation, suggesting that functioning TK pathways are required for estrogen action.

 

动物实验:

 

Mice: MH-85 tumors 5mm in diameter are inoculated s.c. into the right dorsal portion of 4- to 6-week-old male BALB/c nu/nu mice. RG-13022 or RG-14620 in 0.1ml 100% DMSO is injected i.p. twice a day from 1day after MH-85 tumor inoculation.Control animals are given the same vehicle. Tumor sizes are measured once a week under anesthesia with nembutal (0.05mg/g body weight, i.p.) and calculated.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
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    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):