中文名称: Recilisib
英文名称: Recilisib
CAS No: 334969-03-8
分子式: C16H13ClO4S
分子量: 336.79
R10029 Recilisib ≥98%(HPLC) (psaitong)
包装规格:
1mg 5mg in glass bottle
溶解性:
溶于DMSO(3.3mg/mL)
产品描述:

基本信息

产品编号:

R10029

产品名称:

Recilisib

CAS:

334969-03-8

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C16H13ClO4S

溶于液体

-80℃

6个月

分子量:

336.79

-20℃

1个月

化学名: 

ON01210;(E)-4-(2-((4-chlorobenzyl)sulfonyl)vinyl)benzoate

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

 

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.9692mL

14.8460mL

29.6921mL

5mM

0.5938mL

2.9692mL

5.9384mL

10mM

0.2969mL

1.4846mL

2.9692mL

 

生物活性

产品描述

一种辐射防护剂,可改变接受放射治疗的癌细胞中的细胞周期分布模式,已被确认为辐射防护研究的潜在候选物。

靶点

Akt

PI3K

 

体外研究

Recilisib Sodium (up to 50µM) shows a normal distribution of cells throughout the cell cycle,with a slight reduction in the number of cells in S-phase at 50µM.Continuous exposure of Recilisib Sodium (100µM) does not result in cell death.Recilisib Sodium does not inhibit the ability of human bone marrow to form colonies in methylcellulose at either timepoint.Recilisib Sodium treatment does not inhibit the colony forming potential of human bone marrow cells.Recilisib Sodium provides dose dependent protection of human bone marrow cells at all three doses of IR.Recilisib Sodium activates the phosphorylation of AKT and GSK3α/β in HFL cells.Recilisib Sodium increases PI3K activity in HFL-1 cells and murine bone marrow cells in response to radiation exposure.Recilisib Sodium treatment in combination with radiation alters the MAPK signaling pathway.

体内研究

Recilisib Sodium (500mg/kg) significantly increases the rate of recovery and differentiation of primitive bone marrow myeloid progenitor cells in mice.Recilisib Sodium in combination with radiation reduces CFU numbers in mice,but the Recilisib Sodium-treated mice consistently retain a capability to form differentiated colonies. Recilisib Sodium treated mice have a progenitor cell population that is never completely depleted by radiation exposure.

 

推荐实验方法(仅供参考)

激酶实验:

PI3-kinase assays are performed using exponentially growing HFL-1 or freshly harvested murine bone marrow cells that are treated with increasing concentrations of Recilisib Sodium for 2 hours and then irradiated with 10 Gy IR.These cells are then returned to the incubator for 2 to 24 hours and lysed in HEPES pH 7.5 lysis buffer.PI-3K is immunoprecipitated using an antiPI3 Kinas polyclonal antibody for 2 hours at 4℃.Protein A/G PLUS-Agarose is incubated with immunoprecipitates for 8-16 hours at 4℃ and the resulting immunoprecipitates washed with twice HEPES pH 7.5 lysis buffer and once with the kinase buffer (20mM Tris pH 7.5,1mM EGTA,10mM MgCl2,2mM DTT,0.01% NP-40).L-α-Phosphatidylinositol (12.5mM) and ATP (10µM) are added to the kinase buffer (60µL per sample) and incubated at 30℃ for 30 minutes.The reaction is stopped by addition of 100µL of 1N HCl and extracted by addition of 200µL CHCl3/CH3OH (1:1).The extracted samples are vortexed,centrifuged and the lower organic phases containing phospholipids are dried at 27℃ for 2 hours.The dried samples are resuspended in 10µL of PI-4-P standard (0.5mL CHCl3,0.5mL CH3OH,2.5µL HCl) and spotted on TLC plates (VWR).The spotted plate is subjected to thin layer chromatography in CHCl3/CH3OH/NH4OH (40:40:15).The TLC plate is dried and subjected to autoradiography.

 

细胞实验:

 

For cytotoxicity assays,cells (1.0×105 cells/mL HPGM) are treated with various concentrations of Recilisib Sodium or vehicle without radiation treatment for 2 or 24 hours.The cells are washed and plated into methocult using gridded dishes.The total number of colony forming units (CFUs) is determined 14 days post-plating by microscopic observation using an Olympus IMT-2 microscope.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):