| 中文名称: | 硝酸毛果芸香碱 | ||||
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| 英文名称: | Pilocarpine nitrate | ||||
| 别名: | 硝酸毛果芸香碱 Pilocarpine nitrate | ||||
| CAS No: | 148-72-1 | 分子式: | C11H17N3O5 | 分子量: | 271.27 |
| CAS No: | 148-72-1 | ||||
| 分子式: | C11H17N3O5 | ||||
| 分子量: | 271.27 | ||||
| EINEC: | 205-723-7 | ||||
| EINEC: | 205-723-7 | ||||
基本信息
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产品编号： |
P60018 |
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产品名称： |
Pilocarpine nitrate salt |
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CAS: |
148-72-1 |
储存条件 |
粉末 |
室温 |
四年 |
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分子式： |
溶于液体 |
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分子量 |
271.27 |
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化学名： |
(3S,4R)-3-Ethyl-4-((1-methyl-1H-imidazol-5-yl)methyl)dihydrofuran-2(3H)-one nitrate |
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生物活性
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产品描述 |
一种胆碱能毒蕈碱激动剂，在动物模型中可用于诱发癫痫。 |
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靶点 |
M3 muscarinic receptor |
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体外研究 |
To evaluate the cytotoxicity of Pilocarpine, the morphology and viability of human corneal stromal (HCS) cells are examined by light microscopy and MTT assay, respectively. Morphological observations show that HCS cells exposed to Pilocarpine at a concentration from 0.625 to 20g/L exhibit dose- and time-dependent proliferation retardation and morphological abnormality such as cellular shrinkage, cytoplasmic vacuolation, detachment from culture matrix, and eventually death, while no obvious difference is observed between those exposed to Pilocarpine below the concentration of 0.625g/L and controls. Results of MTT assay reveal that the cell viability of HCS cells decrease with time and concentration after exposing to Pilocarpine above the concentration of 0.625g/L (P<0.01 or 0.05), while that of HCS cells treated with Pilocarpine below the concentration of 0.625g/L show no significant difference to controls. The partial muscarinic agonist, Pilocarpine, evokes concentration-dependent relaxation with an EC50 of 2.4mM in isolated segments of rat tail artery that were constricted with Penylephrine (10 to 200nM) |
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体内研究 |
The Pilocarpine-induced saliva secretion of the control rats (CN) and exercised (EX) rats is examined. A significantly greater amount of saliva is induced by Pilocarpine in the EX rats than in the CN rats (P<0.01). Conversely, the Na+ concentration in the saliva of the EX rats is significantly lower than that of the CN rats (P<0.05) |
推荐实验方法(仅供参考)
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Cell Assay |
Cell viability is determined by MTT assay. Briefly, HCS cells are inoculated into a 96-well culture plate (Nunc) at a density of 1×104 cells/100µL/well, and are cultured and treated. At a 4h interval, the Pilocarpine (0.625 to 20g/L)-containing medium is replaced entirely with 100µL serum-free DMEM/F12 medium containing 1.0g/L MTT, and the cells are incubated at 37°C in the dark for 4h. After the MTT-containing medium is discarded with caution, 150µL DMSO is added to dissolve the produced formazan crystals at 37°C in the dark for 15 min, and the absorbance at 490nM is measured with a Multiskan GO microplate reader |
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Animal Administration |
Rats Male, 10-week-old Wistar rats are assigned to one of two groups, exercise (EX, n=6) and control (CN, n=6). The EX rats are kept for 40 days in cages with a running wheel (SN-451), allowing them to undertake voluntary exercise, while the CN rats are kept in cages with the running wheel locked. On the 40th day, Pilocarpine-induced saliva is measured as follows. Briefly, the rats are anesthetized, preweighed cotton was placed in their mouths sublingually, and Pilocarpine (0.5mg/kg) is intraperitoneally injected to induce saliva secretion. Each cotton ball is then changed every 10 min for 1 h. The collected cotton balls are weighed again, and the mass of saliva secreted is calculated by subtracting the initial from the final weight. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
