中文名称: 尼日利亚菌素钠盐
英文名称: Nigericin sodium salt
CAS No: 28643-80-3
分子式: C40H67NaO11
分子量: 746.95
N10364 尼日利亚菌素钠盐 ≥98% (psaitong)
包装规格:
5mg 10mg 25mg 50mg 100mg in glass bottle
溶解性:
溶于DMSO(5 mg/mL 超声 加热)
产品描述:

基本信息

产品编号:

N10364

产品名称:

Nigericin sodium salt

CAS:

28643-80-3

 

储存条件

粉末

-20℃

四年

分子式:

C40H67NaO11

溶于液体

-80℃(密封储存,防潮)

6个月

分子量:

746.95

-20℃(密封储存,防潮)

1个月

化学名: 

(R)-2-((2R,3S,6R)-6-(((2S,4R,5R,7R,9R,10R)-2-((2S,2'R,3'S,5R,5'R)-5'-((2S,3S,5R,6R)-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyltetrahydro-2H-pyran-2-yl)-2,3'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-9-methoxy-2,4,10-trimethyl-1,6-dioxaspiro[4.5]decan-7-yl)methyl)-3-methyltetrahydro-2H-pyran-2-yl)propanoate

Solubility (25°C):

 

体外:

 

DMSO

Insoluble

Ethanol

100mg/mL (133.87mM)

Water

Insoluble

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

1.3388mL

6.6940mL

13.3880mL

5mM

0.2678mL

1.3388mL

2.6776mL

10mM

0.1339mL

0.6694mL

1.3388mL

 

生物活性

产品描述

一种从吸水链霉菌中得到的抗生素。

靶点

Potassium Channel

NOD-like Receptor (NLR)

Bacterial

Antibiotic

 

体外研究

Nigericin (0.1µM) decreases inhibits proliferation and clonogenicity of H460 lung cancer cells in a dose dependent manner.Nigericin inhibits migration and invasion of H460 lung cancer cells.Nigericin (0.1-10nM) has apparently a dual effect on cell volume,that is a shrinking effect at lower Nigericin concentrations and a swelling effect at higher concentrations.Nigericin (0.1-1nM) significantly decreases cytosolic pH (pHi),and slightly increases the pHi at 5 and 10 nM.Nigericin exhibits higher toxicity on S18 cells than S26 cells,with IC50 of 2.03±0.55μM and 4.77±2.35μM,respectively.Nigericin can selectively kill cancer stem cells in NPC in vitro.Nigericin dramatically reduces the migration ability of S18 and HONE-1 cells.Nigericin exhibits gteat toxicity for the HT29 and SW116 cell line with IC50 of 12.92±0.25μmol and 15.86±0.18μmol.Nigericin also shows a decreased ability to form colonies under anchorage-independent conditions in a standard soft agar assay.

体内研究

Ngericin (4mg/kg,i.p.) significantly reduces tumor growth and acts synergistically with the chemotherapeutic agent DDP,as shown by the tumor volumes.Nigericin markedly decreases Bmi-1 in vivo.Overexpression of Bmi-1 partially restores CSC content and metastatic ability of NPC cells under Nigericin treatment.The downregulation of Bmi-1 may be involved in the inhibitory effect of Nigericin on CSCs in NPC.

 

推荐实验方法(仅供参考)

细胞实验:

 

For RCCs,cells (appr 2000 cells/well) are plated in 96-well cell-culture microplates and incubated over nigericinht in complete media (CM)-RPMI 1640 supplemented with 5% FBS and 2mM l-glutamine.Cells are then exposed to the appropriate concentration of drug or vehicle for 72h.For PPSS,cells (appr 500 cells/well) are plated in 96-well cell-culture microplates incubated over Nigericinht in CM to allow them to adhere and then maintained in serum-free media for 7-8 days and then treated with the appropriate concentration of drug or vehicle for 72h in SFM.Cell viability for cells growing under RCCs and PPSS are evaluated by the MTT assay.The absorbance of solubilized formazan is read at 570nm using ELISA (enzyme-linked immunosorbent assay) reader.In all cases,the highest concentration of DMSO is used in the control and this concentration is maintained below 0.001% (v/v).This DMSO concentration does not show any significant antiproliferative effect on the cell line in a short-term assay.

 

动物实验:

 

Mice

The S18 cells are injected near the scapula of the nude mice.Nine days after injection,the mice are randomly divided into four groups with six animals each (control,DDP,Nigericin and DDP combined with Nigericin).DDP (2.5mg/kg) is injected intraperitoneally for five continuous days and nigericin (4mg/kg) is administrated intraperitoneally every two days.Tumor length and width are measured with a vernier caliper every other day.Tumor volume is calculated using the formula V=0.5×(length×width2).The body weights of the mice are recorded every two days.Mice are humanely euthanized when the tumor volume reach 2000 mm3.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):