中文名称: | NG-52 促销 | ||||
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英文名称: | NG-52 | ||||
别名: | NG-52 NG52;Compound 52;2-((6-((3-chlorophenyl)amino)-9-isopropyl-9H-purin-2-yl)amino)ethan-1-ol | ||||
CAS No: | 212779-48-1 | 分子式: | C16H19ClN6O | 分子量: | 346.82 |
CAS No: | 212779-48-1 | ||||
分子式: | C16H19ClN6O | ||||
分子量: | 346.82 |
基本信息
产品编号:N10153 |
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产品名称:NG-52 |
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CAS: |
212779-48-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
346.82 |
-20℃ |
一个月 |
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化学名: |
2-(2-HYDROXYETHYLAMINO)-6-(3-CHLOROANILINO)-9-ISOPROPYLPURINE |
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Solubility (25°C) |
体外 |
DMSO |
69mg/mL (198.95mM) |
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Ethanol |
23mg/mL (66.31mM) |
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Water |
Insoluble |
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体内 |
现配现用 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.8834mL |
14.4171mL |
28.8342mL |
5mM |
0.5767mL |
2.8834mL |
5.7668mL |
10mM |
0.2883mL |
1.4417mL |
2.8834mL |
50mM |
0.0577mL |
0.2883mL |
0.5767mL |
生物活性
产品描述 |
一种可渗透入细胞的可逆的非ATP竞争性的细胞周期调节激酶Cdc28p和Pho85p激酶抑制剂,IC50分别为7μM和2μM。 |
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靶点/IC50 |
Pho85p |
Cdc28p |
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2μM |
7μM |
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体外研究 |
NG 52 (Compound 52) inhibits growth in a drug-sensitized yeast strain (S. cerevisiae) with a GI50 of 30μM. NG 52 is active against cdc2-cyclin B with an IC50 value of 340nM. NG 52 dose-dependently inhibits the proliferation of glioma U87 and U251 cell lines with GI50 values of 7.8μM and 5.2μM,respectively, meanwhile it potently inhibits the proliferation of primary glioma cells. NG 52 (12.5-50μM) effectively inhibits the phosphorylation of PDHK1 at Thr338 site and the phosphorylation of PDH at Ser293 site in U87 and U251 cells, resulting in more pyruvic acid entering the Krebs cycle with increased production of ATP and ROS. NG 52 can reverse the Warburg effect by enhancing the activity of pyruvate dehydrogenase (PDH) through inhibiting the activity of PGK1, and switched cellular glucose metabolism from anaerobic mode to aerobic mode. Cell Proliferation Assay |
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Cell Line: |
Glioma U87 and U251 cells |
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Concentration: |
0μM, 12.5μM, 25μM, 50μM |
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Incubation Time: |
6 days |
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Result: |
Potently inhibited the proliferation of primary glioma cells. |
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Western Blot Analysis |
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Cell Line: |
Glioma U87 and U251 cells |
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Concentration: |
0μM, 12.5μM, 25μM, 50μM |
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Incubation Time: |
12 hours or 24 hours |
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Result: |
Potently inhibited the proliferation of primary glioma cells. |
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体内研究 |
NG 52 (50-150mg/kg; oral administration; daily; for 13 days) treatment dose-dependently suppresses the growth of glioma xenograft . |
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Animal Model: |
Female nu/nu mice (5-week-old) injected with glioma cells |
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Dosage: |
50mg/kg, 100mg/kg, 150mg/kg |
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Administration: |
Oral administration; daily; for 13 days |
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Result: |
Dose-dependently suppressed the growth of glioma xenograft. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )